Combined protein A imprinting and cryogelation for production of spherical affinity material
(2019) In Biomedical Chromatography 33(10).- Abstract
Cryogels have been demonstrated to be efficient when applied for protein isolation. Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs). These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized... (More)
Cryogels have been demonstrated to be efficient when applied for protein isolation. Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs). These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized and the separation properties were evaluated with regard to both the target protein and whole cells with target protein exposed on the cell surface. The maximum protein A adsorption was 18.1 mg/g of wet cryogel beads. The selectivity coefficient of protein A-imprinted cryogel beads for protein A was 5.44 and 12.56 times greater than for the Fc fragment of IgG and protein G, respectively.
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- author
- Aslıyüce, Sevgi ; Mattiasson, Bo LU and Denizli, Adil
- organization
- publishing date
- 2019-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cryogel beads, molecular imprinting technology, protein A, radical polymerization
- in
- Biomedical Chromatography
- volume
- 33
- issue
- 10
- article number
- e4605
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:31140195
- scopus:85068522923
- ISSN
- 0269-3879
- DOI
- 10.1002/bmc.4605
- language
- English
- LU publication?
- yes
- id
- 56621740-4e3b-4b6d-bf95-d06cb38b042f
- date added to LUP
- 2019-07-17 15:05:50
- date last changed
- 2024-04-16 16:31:24
@article{56621740-4e3b-4b6d-bf95-d06cb38b042f,
abstract = {{<p>Cryogels have been demonstrated to be efficient when applied for protein isolation. Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs). These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized and the separation properties were evaluated with regard to both the target protein and whole cells with target protein exposed on the cell surface. The maximum protein A adsorption was 18.1 mg/g of wet cryogel beads. The selectivity coefficient of protein A-imprinted cryogel beads for protein A was 5.44 and 12.56 times greater than for the Fc fragment of IgG and protein G, respectively.</p>}},
author = {{Aslıyüce, Sevgi and Mattiasson, Bo and Denizli, Adil}},
issn = {{0269-3879}},
keywords = {{cryogel beads; molecular imprinting technology; protein A; radical polymerization}},
language = {{eng}},
number = {{10}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biomedical Chromatography}},
title = {{Combined protein A imprinting and cryogelation for production of spherical affinity material}},
url = {{http://dx.doi.org/10.1002/bmc.4605}},
doi = {{10.1002/bmc.4605}},
volume = {{33}},
year = {{2019}},
}