Metalloproteases and serineproteases are involved in the cleavage of the two tumour necrosis factor (TNF) receptors to soluble forms in the myeloid cell lines U-937 and THP-1
(1995) In Scandinavian Journal of Immunology 42(4). p.24-418- Abstract
The proteolytic processing of the two TNF receptors (TNF-R55 and TNF-R75) into soluble forms was investigated in the myeloid cell lines U-937 and THP-1. Phorbol myristate acetate (PMA) rapidly stimulated release of soluble forms of both TNF-receptors. Incubations were made with PMA and protease inhibitors directed against different target protease groups. The serineprotease inhibitors TPCK and dichloroisocoumarin and the metalloprotease inhibitor 1,10-phenanthroline reduced PMA-induced release of both soluble receptor forms with about 60-70%. Furthermore, 1,10-phenanthroline also reduced PMA-induced down-regulation of TNF-receptors in both cell lines as judged by TNF-binding to cells. Reduced down-regulation and TNF-receptor shedding by... (More)
The proteolytic processing of the two TNF receptors (TNF-R55 and TNF-R75) into soluble forms was investigated in the myeloid cell lines U-937 and THP-1. Phorbol myristate acetate (PMA) rapidly stimulated release of soluble forms of both TNF-receptors. Incubations were made with PMA and protease inhibitors directed against different target protease groups. The serineprotease inhibitors TPCK and dichloroisocoumarin and the metalloprotease inhibitor 1,10-phenanthroline reduced PMA-induced release of both soluble receptor forms with about 60-70%. Furthermore, 1,10-phenanthroline also reduced PMA-induced down-regulation of TNF-receptors in both cell lines as judged by TNF-binding to cells. Reduced down-regulation and TNF-receptor shedding by 1,10-phenanthroline was reversed by Zn2+, indicating involvement of a Zn(2+)-dependent metalloprotease. Thus, both serine proteases and metalloproteases are involved in the processing of TNF-receptors.
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- author
- Björnberg, F LU ; Lantz, M LU and Gullberg, U LU
- organization
- publishing date
- 1995-10
- type
- Contribution to journal
- publication status
- published
- keywords
- Enzyme Inhibitors, Humans, Metalloendopeptidases, Receptors, Tumor Necrosis Factor, Serine Endopeptidases, Signal Transduction, Tetradecanoylphorbol Acetate, Tumor Cells, Cultured
- in
- Scandinavian Journal of Immunology
- volume
- 42
- issue
- 4
- pages
- 7 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0028971793
- pmid:7569774
- ISSN
- 0300-9475
- language
- English
- LU publication?
- yes
- id
- 5f154dad-bab6-4068-a796-c36a2a912a0d
- date added to LUP
- 2016-06-01 10:08:25
- date last changed
- 2024-01-04 07:47:49
@article{5f154dad-bab6-4068-a796-c36a2a912a0d, abstract = {{<p>The proteolytic processing of the two TNF receptors (TNF-R55 and TNF-R75) into soluble forms was investigated in the myeloid cell lines U-937 and THP-1. Phorbol myristate acetate (PMA) rapidly stimulated release of soluble forms of both TNF-receptors. Incubations were made with PMA and protease inhibitors directed against different target protease groups. The serineprotease inhibitors TPCK and dichloroisocoumarin and the metalloprotease inhibitor 1,10-phenanthroline reduced PMA-induced release of both soluble receptor forms with about 60-70%. Furthermore, 1,10-phenanthroline also reduced PMA-induced down-regulation of TNF-receptors in both cell lines as judged by TNF-binding to cells. Reduced down-regulation and TNF-receptor shedding by 1,10-phenanthroline was reversed by Zn2+, indicating involvement of a Zn(2+)-dependent metalloprotease. Thus, both serine proteases and metalloproteases are involved in the processing of TNF-receptors.</p>}}, author = {{Björnberg, F and Lantz, M and Gullberg, U}}, issn = {{0300-9475}}, keywords = {{Enzyme Inhibitors; Humans; Metalloendopeptidases; Receptors, Tumor Necrosis Factor; Serine Endopeptidases; Signal Transduction; Tetradecanoylphorbol Acetate; Tumor Cells, Cultured}}, language = {{eng}}, number = {{4}}, pages = {{24--418}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Immunology}}, title = {{Metalloproteases and serineproteases are involved in the cleavage of the two tumour necrosis factor (TNF) receptors to soluble forms in the myeloid cell lines U-937 and THP-1}}, volume = {{42}}, year = {{1995}}, }