Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging
(2005) Photon Migration and Diffuse-Light Imaging II 5859(1). p.1-585906- Abstract
- A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a... (More)
- A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/616409
- author
- Svensson, Jenny LU ; Garofalakis, A ; Meyer, H ; Forster, Florian LU ; Ripoll, J and Andersson-Engels, Stefan LU
- organization
- publishing date
- 2005
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- 2 cm, yellow fluorescence, fluorophores, red fluorescence, phantom measurements, optical tomography, fluorescent light detection, fluorescent probe, deep-lying tumors, fluorescent object, tissue imaging, depth resolution, fluorescence spectroscopy, tissue phantoms
- host publication
- Proceedings of the SPIE - The International Society for Optical Engineering
- volume
- 5859
- issue
- 1
- pages
- 1 - 585906
- publisher
- SPIE
- conference name
- Photon Migration and Diffuse-Light Imaging II
- conference location
- Munich, Germany
- conference dates
- 2005-06-12
- external identifiers
-
- scopus:28844440135
- ISSN
- 1996-756X
- 0277-786X
- DOI
- 10.1117/12.632826
- language
- English
- LU publication?
- yes
- id
- 64e293bd-fb3e-4e38-9b4f-40a1806c949f (old id 616409)
- date added to LUP
- 2016-04-01 12:17:16
- date last changed
- 2024-01-08 15:05:46
@inproceedings{64e293bd-fb3e-4e38-9b4f-40a1806c949f, abstract = {{A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object}}, author = {{Svensson, Jenny and Garofalakis, A and Meyer, H and Forster, Florian and Ripoll, J and Andersson-Engels, Stefan}}, booktitle = {{Proceedings of the SPIE - The International Society for Optical Engineering}}, issn = {{1996-756X}}, keywords = {{2 cm; yellow fluorescence; fluorophores; red fluorescence; phantom measurements; optical tomography; fluorescent light detection; fluorescent probe; deep-lying tumors; fluorescent object; tissue imaging; depth resolution; fluorescence spectroscopy; tissue phantoms}}, language = {{eng}}, number = {{1}}, pages = {{1--585906}}, publisher = {{SPIE}}, title = {{Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging}}, url = {{http://dx.doi.org/10.1117/12.632826}}, doi = {{10.1117/12.632826}}, volume = {{5859}}, year = {{2005}}, }