Applications of optical DNA mapping in microbiology
Bogas, Diana ; Nyberg, Lena ; Pacheco, Rui ; Azevedo, Nuno F. ; Beech, Jason P. LU ; Gomila, Margarita ; Lalucat, Jorge ; Manaia, Célia M. ; Nunes, Olga C. and Tegenfeldt, Jonas O. LU
, et al.
(2017)
In BioTechniques
62(6).
p.255-267
- Abstract
Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the... (More)
Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.
(Less)
- author
- Bogas, Diana
; Nyberg, Lena
; Pacheco, Rui
; Azevedo, Nuno F.
; Beech, Jason P.
LU
; Gomila, Margarita
; Lalucat, Jorge
; Manaia, Célia M.
; Nunes, Olga C.
and Tegenfeldt, Jonas O.
LU
, et al. (More)
- Bogas, Diana
; Nyberg, Lena
; Pacheco, Rui
; Azevedo, Nuno F.
; Beech, Jason P.
LU
; Gomila, Margarita
; Lalucat, Jorge
; Manaia, Célia M.
; Nunes, Olga C.
; Tegenfeldt, Jonas O.
LU
and Westerlund, Fredrik
(Less)
- organization
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- DNA-based identification, Imaging technique, Lab-on-a chip, Microbiological clinical diagnosis, Optical DNA mapping
- in
- BioTechniques
- volume
- 62
- issue
- 6
- pages
- 13 pages
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:28625155
- wos:000403563300004
- scopus:85020897312
- ISSN
- 0736-6205
- DOI
- 10.2144/000114555
- language
- English
- LU publication?
- yes
- id
- 639288bb-26a0-4ef2-976e-380f8699d475
- date added to LUP
- 2017-07-05 09:35:54
- date last changed
- 2024-01-14 00:17:58
@article{639288bb-26a0-4ef2-976e-380f8699d475,
abstract = {{<p>Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.</p>}},
author = {{Bogas, Diana and Nyberg, Lena and Pacheco, Rui and Azevedo, Nuno F. and Beech, Jason P. and Gomila, Margarita and Lalucat, Jorge and Manaia, Célia M. and Nunes, Olga C. and Tegenfeldt, Jonas O. and Westerlund, Fredrik}},
issn = {{0736-6205}},
keywords = {{DNA-based identification; Imaging technique; Lab-on-a chip; Microbiological clinical diagnosis; Optical DNA mapping}},
language = {{eng}},
number = {{6}},
pages = {{255--267}},
publisher = {{Informa Healthcare}},
series = {{BioTechniques}},
title = {{Applications of optical DNA mapping in microbiology}},
url = {{http://dx.doi.org/10.2144/000114555}},
doi = {{10.2144/000114555}},
volume = {{62}},
year = {{2017}},
}