Production of Cystatin C Wild Type and Stabilized Mutants
(2010) In EJIFCC 20(4). p.70-166- Abstract
Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.
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https://lup.lub.lu.se/record/640ac852-1071-4ed3-b7de-ccfdca16f689
- author
- Pollak, Joanna LU ; Szymanska, Aneta ; Lindström, Veronica LU and Grubb, Anders LU
- organization
- publishing date
- 2010-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Journal Article
- in
- EJIFCC
- volume
- 20
- issue
- 4
- pages
- 5 pages
- publisher
- International Federation of Clinical Chemistry and Laboratory Medicine
- external identifiers
-
- pmid:27683345
- ISSN
- 1650-3414
- language
- English
- LU publication?
- yes
- id
- 640ac852-1071-4ed3-b7de-ccfdca16f689
- date added to LUP
- 2016-11-22 19:02:22
- date last changed
- 2018-11-21 21:27:33
@article{640ac852-1071-4ed3-b7de-ccfdca16f689, abstract = {{<p>Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.</p>}}, author = {{Pollak, Joanna and Szymanska, Aneta and Lindström, Veronica and Grubb, Anders}}, issn = {{1650-3414}}, keywords = {{Journal Article}}, language = {{eng}}, number = {{4}}, pages = {{70--166}}, publisher = {{International Federation of Clinical Chemistry and Laboratory Medicine}}, series = {{EJIFCC}}, title = {{Production of Cystatin C Wild Type and Stabilized Mutants}}, volume = {{20}}, year = {{2010}}, }