Standardizing evaluation of sarcoma proliferation - higher Ki-67 expression in the tumor periphery than the center
(2007) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 115(6). p.707-712- Abstract
- Soft tissue sarcomas often present as large and histopathologically heterogenous tumors. Proliferation has repeatedly been identified as a prognostic factor and immunostaining for Ki-67 represents the most commonly used proliferation marker. There is, however, a lack of consensus on how to evaluate Ki-67 staining regarding optimal cut-off levels, selection of tumor areas, and the number of tumor cells to evaluate. We assessed the impact of targeting peripheral versus central tumor areas using tissue microarray-based staining for Ki-67 throughout the tumor diameter in 25 leiomyosarcomas. In 18/25 tumors, Ki-67 expression was higher in the tumor periphery. If 10% staining tumor nuclei was used as cut-off and the maximal Ki-67 staining... (More)
- Soft tissue sarcomas often present as large and histopathologically heterogenous tumors. Proliferation has repeatedly been identified as a prognostic factor and immunostaining for Ki-67 represents the most commonly used proliferation marker. There is, however, a lack of consensus on how to evaluate Ki-67 staining regarding optimal cut-off levels, selection of tumor areas, and the number of tumor cells to evaluate. We assessed the impact of targeting peripheral versus central tumor areas using tissue microarray-based staining for Ki-67 throughout the tumor diameter in 25 leiomyosarcomas. In 18/25 tumors, Ki-67 expression was higher in the tumor periphery. If 10% staining tumor nuclei was used as cut-off and the maximal Ki-67 staining section in the tumor periphery was considered, 21/25 tumors would have been classified as highly proliferative compared to 14/25 if the tumor center had been analyzed. Similar results were obtained also when higher cut-off levels were used and if the mean expression rather than the maximal expression was considered and the differences were neither caused by necrosis nor by hypoxia (assessed as HIF-1 alpha expression). Our findings suggest that the determination of proliferation in soft tissue sarcomas should be standardized for clinical application of Ki-67 as a prognostic marker. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/651202
- author
- Fernebro, Josefin LU ; Engellau, Jacob LU ; Persson, Annette LU ; Rydholm, Anders LU and Nilbert, Mef LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- leiomyosarcoma, soft tissue sarcoma, Ki-67, proliferation, variability, intratumor
- in
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- volume
- 115
- issue
- 6
- pages
- 707 - 712
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000246910700003
- scopus:34249810648
- ISSN
- 1600-0463
- DOI
- 10.1111/j.1600-0463.2007.apm_650.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Oncology, MV (013035000), Pathology, (Lund) (013030000), Department of Orthopaedics (Lund) (013028000)
- id
- cdd0ab7e-c2f0-4e81-8800-c89531690930 (old id 651202)
- date added to LUP
- 2016-04-01 11:45:28
- date last changed
- 2022-02-25 20:51:42
@article{cdd0ab7e-c2f0-4e81-8800-c89531690930,
abstract = {{Soft tissue sarcomas often present as large and histopathologically heterogenous tumors. Proliferation has repeatedly been identified as a prognostic factor and immunostaining for Ki-67 represents the most commonly used proliferation marker. There is, however, a lack of consensus on how to evaluate Ki-67 staining regarding optimal cut-off levels, selection of tumor areas, and the number of tumor cells to evaluate. We assessed the impact of targeting peripheral versus central tumor areas using tissue microarray-based staining for Ki-67 throughout the tumor diameter in 25 leiomyosarcomas. In 18/25 tumors, Ki-67 expression was higher in the tumor periphery. If 10% staining tumor nuclei was used as cut-off and the maximal Ki-67 staining section in the tumor periphery was considered, 21/25 tumors would have been classified as highly proliferative compared to 14/25 if the tumor center had been analyzed. Similar results were obtained also when higher cut-off levels were used and if the mean expression rather than the maximal expression was considered and the differences were neither caused by necrosis nor by hypoxia (assessed as HIF-1 alpha expression). Our findings suggest that the determination of proliferation in soft tissue sarcomas should be standardized for clinical application of Ki-67 as a prognostic marker.}},
author = {{Fernebro, Josefin and Engellau, Jacob and Persson, Annette and Rydholm, Anders and Nilbert, Mef}},
issn = {{1600-0463}},
keywords = {{leiomyosarcoma; soft tissue sarcoma; Ki-67; proliferation; variability; intratumor}},
language = {{eng}},
number = {{6}},
pages = {{707--712}},
publisher = {{John Wiley & Sons Inc.}},
series = {{APMIS : acta pathologica, microbiologica, et immunologica Scandinavica}},
title = {{Standardizing evaluation of sarcoma proliferation - higher Ki-67 expression in the tumor periphery than the center}},
url = {{http://dx.doi.org/10.1111/j.1600-0463.2007.apm_650.x}},
doi = {{10.1111/j.1600-0463.2007.apm_650.x}},
volume = {{115}},
year = {{2007}},
}