Expression of MMP-10 in lung cancer
(2007) In Anticancer research 27(4C). p.2791-2795- Abstract
- Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and... (More)
- Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and in the adjacent normal tissues. The ratio of mRNA levels in the tumor tissues compared to the adjacent normal tissues followed the pattern GAPDH > beta-actin > 18S rRNA. Thereafter, we chose 18S rRNA as the reference gene for MMP-10 mRNA level determinations. MMP-10 mRNA levels in tumor tissues were significantly lower than those in the adjacent normal tissues (p = 0.0423). However, the MMP- 10 protein levels were higher in the tumor tissues than in the adjacent normal tissues (p=0.0055). The MMP-10 mRNA level was positively-correlated to the MMP-10 protein level in tumor tissues (r=0.4672, p=0.0161), but this correlation was not seen in the adjacent normal tissues (r=-0.0030, p=0.9891). Conclusion: There were no statistical differences in MMP-10 mRNA levels and protein levels in relation to patient's gender, age, tumor stages, tumor size, lymph node metastasis or tumor histological type. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/686802
- author
- Zhang, Xiaoying ; Zhu, Shaowei ; Luo, Guanghua ; Zheng, Lu ; Wei, Jiang ; Zhu, Jiang ; Mu, Qingfeng and Xu, Ning LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- real-time RT-PCR, endogenous reference gene, MMP-10, NSCLC, immunohistochemistry
- in
- Anticancer research
- volume
- 27
- issue
- 4C
- pages
- 2791 - 2795
- publisher
- International Institute of Cancer Research
- external identifiers
-
- wos:000248546100034
- scopus:34547784153
- ISSN
- 1791-7530
- language
- English
- LU publication?
- yes
- id
- 179e4c31-b78d-41b3-9f85-af01fab8c280 (old id 686802)
- date added to LUP
- 2016-04-01 11:36:45
- date last changed
- 2022-02-10 18:57:16
@article{179e4c31-b78d-41b3-9f85-af01fab8c280,
abstract = {{Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and in the adjacent normal tissues. The ratio of mRNA levels in the tumor tissues compared to the adjacent normal tissues followed the pattern GAPDH > beta-actin > 18S rRNA. Thereafter, we chose 18S rRNA as the reference gene for MMP-10 mRNA level determinations. MMP-10 mRNA levels in tumor tissues were significantly lower than those in the adjacent normal tissues (p = 0.0423). However, the MMP- 10 protein levels were higher in the tumor tissues than in the adjacent normal tissues (p=0.0055). The MMP-10 mRNA level was positively-correlated to the MMP-10 protein level in tumor tissues (r=0.4672, p=0.0161), but this correlation was not seen in the adjacent normal tissues (r=-0.0030, p=0.9891). Conclusion: There were no statistical differences in MMP-10 mRNA levels and protein levels in relation to patient's gender, age, tumor stages, tumor size, lymph node metastasis or tumor histological type.}},
author = {{Zhang, Xiaoying and Zhu, Shaowei and Luo, Guanghua and Zheng, Lu and Wei, Jiang and Zhu, Jiang and Mu, Qingfeng and Xu, Ning}},
issn = {{1791-7530}},
keywords = {{real-time RT-PCR; endogenous reference gene; MMP-10; NSCLC; immunohistochemistry}},
language = {{eng}},
number = {{4C}},
pages = {{2791--2795}},
publisher = {{International Institute of Cancer Research}},
series = {{Anticancer research}},
title = {{Expression of MMP-10 in lung cancer}},
volume = {{27}},
year = {{2007}},
}