Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
(2007) In Nature Biotechnology 25(7). p.803-816- Abstract
- The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of... (More)
- The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected. (Less)
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- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Biotechnology
- volume
- 25
- issue
- 7
- pages
- 803 - 816
- publisher
- Nature Publishing Group
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- wos:000247994000032
- scopus:34447295350
- ISSN
- 1546-1696
- DOI
- 10.1038/nbt1318
- language
- English
- LU publication?
- yes
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- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Stem Cell and Pancreas Developmental Biology (013212044)
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- 14a89f54-d7a0-4ca9-ba46-725f0ab0a89a (old id 691452)
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@article{14a89f54-d7a0-4ca9-ba46-725f0ab0a89a,
abstract = {{The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue- nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.}},
author = {{Adewumi, Oluseun and Aflatoonian, Behrouz and Ahrlund-Richter, Lars and Amit, Michal and Andrews, Peter W. and Beighton, Gemma and Bello, Paul A. and Benvenisty, Nissim and Berry, Lorraine S. and Bevan, Simon and Blum, Barak and Brooking, Justin and Chen, Kevin G. and Choo, Andre B. H. and Churchill, Gary A. and Corbel, Marie and Damjanov, Ivan and Draper, Jon S. and Dvorak, Petr and Emanuelsson, Katarina and Fleck, Roland A. and Ford, Angela and Gertow, Karin and Gertsenstein, Marina and Gokhale, Paul J. and Hamilton, Rebecca S. and Hampl, Ales and Healy, Lyn E. and Hovatta, Outi and Hyllner, Johan and Imreh, Marta P. and Itskovitz-Eldor, Joseph and Jackson, Jamie and Johnson, Jacqueline L. and Jones, Mark and Kee, Kehkooi and King, Benjamin L. and Knowles, Barbara B. and Lako, Majlinda and Lebrin, Franck and Mallon, Barbara S. and Manning, Daisy and Mayshar, Yoav and Mckay, Ronald D. G. and Michalska, Anna E. and Mikkola, Milla and Mileikovsky, Masha and Minger, Stephen L. and Moore, Harry D. and Mummery, Christine L. and Nagy, Andras and Nakatsuji, Norio and O'Brien, Carmel M. and Oh, Steve K. W. and Olsson, Cia and Otonkoski, Timo and Park, Kye-Yoon and Passier, Robert and Patel, Hema and Patel, Minal and Pedersen, Roger and Pera, Martin F. and Piekarczyk, Marian S. and Pera, Renee A. Reijo and Reubinoff, Benjamin E. and Robins, Allan J. and Rossant, Janet and Rugg-Gunn, Peter and Schulz, Thomas C. and Semb, Henrik and Sherrer, Eric S. and Siemen, Henrike and Stacey, Glyn N. and Stojkovic, Miodrag and Suemori, Hirofumi and Szatkiewicz, Jin and Turetsky, Tikva and Tuuri, Timo and van den Brink, Steineke and Vintersten, Kristina and Vuoristo, Sanna and Ward, Dorien and Weaver, Thomas A. and Young, Lesley A. and Zhang, Weidong}},
issn = {{1546-1696}},
language = {{eng}},
number = {{7}},
pages = {{803--816}},
publisher = {{Nature Publishing Group}},
series = {{Nature Biotechnology}},
title = {{Characterization of human embryonic stem cell lines by the International Stem Cell Initiative}},
url = {{http://dx.doi.org/10.1038/nbt1318}},
doi = {{10.1038/nbt1318}},
volume = {{25}},
year = {{2007}},
}