Application of enzymes, sodium tripolyphosphate and cation exchange resin for the release of extracellular polymeric substances from sewage sludge - Characterization of the extracted polysaccharides/glycoconjugates by a panel of lectins
(2007) In Journal of Biotechnology 130(3). p.274-281- Abstract
- The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetrical methods and polysaccharides/glycoconjugates identification by the interaction with four immobilized lectins. Bio-sludge from Helsingborg (Sweden) and Damhusaen (Denmark) were used as two case studies for testing enzymatic extractability and thereby to make useful prediction of sludge bio-digestibility. From Helsingborg sludge the enzymes extracted about 40% more of EPS than from Damhusaen. The... (More)
- The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetrical methods and polysaccharides/glycoconjugates identification by the interaction with four immobilized lectins. Bio-sludge from Helsingborg (Sweden) and Damhusaen (Denmark) were used as two case studies for testing enzymatic extractability and thereby to make useful prediction of sludge bio-digestibility. From Helsingborg sludge the enzymes extracted about 40% more of EPS than from Damhusaen. The polysaccharides/glycoconjugates in both sludges maintained the same level, and showed substantial different interaction motifs with lectins panel. Damhusaen enzymatic extracted EPS had an enhanced amount of suspended material that was post-hydrolysed by the use of polygalacturonase and lysozyme resulting in pectin like polymers and petiptidoglycans. Petiptidoglycan is a marker from bacterial cell debris. STPP and cation exchange resin (CER) released different quantities of EPS. The CER released polysaccharides/glycoconjugates had higher molecular weight and stronger affinity towards Concanavalin A than the one released by the action of STPP. Independent of the extraction conditions, STPP released elevated amounts of polyvalent cations and humic substances in contrast to the very low amounts of released by CER. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/691830
- author
- Wawrzynczyk, Joanna LU ; Szewczyk, Emil LU ; Norrlöw, Olof LU and Dey, Estera LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cation exchange resin, bio-sludge, enzymes, extracellular polymeric, substances, lectin, sodium tripolyphosphate
- in
- Journal of Biotechnology
- volume
- 130
- issue
- 3
- pages
- 274 - 281
- publisher
- Elsevier
- external identifiers
-
- wos:000248109600009
- scopus:34250644419
- pmid:17543410
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2007.04.005
- language
- English
- LU publication?
- yes
- id
- b1c7a9cf-98c1-4263-a2b7-7755219e3975 (old id 691830)
- date added to LUP
- 2016-04-01 12:21:39
- date last changed
- 2022-01-27 02:43:04
@article{b1c7a9cf-98c1-4263-a2b7-7755219e3975, abstract = {{The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetrical methods and polysaccharides/glycoconjugates identification by the interaction with four immobilized lectins. Bio-sludge from Helsingborg (Sweden) and Damhusaen (Denmark) were used as two case studies for testing enzymatic extractability and thereby to make useful prediction of sludge bio-digestibility. From Helsingborg sludge the enzymes extracted about 40% more of EPS than from Damhusaen. The polysaccharides/glycoconjugates in both sludges maintained the same level, and showed substantial different interaction motifs with lectins panel. Damhusaen enzymatic extracted EPS had an enhanced amount of suspended material that was post-hydrolysed by the use of polygalacturonase and lysozyme resulting in pectin like polymers and petiptidoglycans. Petiptidoglycan is a marker from bacterial cell debris. STPP and cation exchange resin (CER) released different quantities of EPS. The CER released polysaccharides/glycoconjugates had higher molecular weight and stronger affinity towards Concanavalin A than the one released by the action of STPP. Independent of the extraction conditions, STPP released elevated amounts of polyvalent cations and humic substances in contrast to the very low amounts of released by CER.}}, author = {{Wawrzynczyk, Joanna and Szewczyk, Emil and Norrlöw, Olof and Dey, Estera}}, issn = {{1873-4863}}, keywords = {{cation exchange resin; bio-sludge; enzymes; extracellular polymeric; substances; lectin; sodium tripolyphosphate}}, language = {{eng}}, number = {{3}}, pages = {{274--281}}, publisher = {{Elsevier}}, series = {{Journal of Biotechnology}}, title = {{Application of enzymes, sodium tripolyphosphate and cation exchange resin for the release of extracellular polymeric substances from sewage sludge - Characterization of the extracted polysaccharides/glycoconjugates by a panel of lectins}}, url = {{http://dx.doi.org/10.1016/j.jbiotec.2007.04.005}}, doi = {{10.1016/j.jbiotec.2007.04.005}}, volume = {{130}}, year = {{2007}}, }