Gamma-tubulin coordinates nuclear envelope assembly around chromatin
(2016) In Heliyon 2(9).- Abstract
The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a... (More)
The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.
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- author
- Rossello Castillo, Catalina Ana LU ; Lindström, Lisa LU ; Glindre, Johan LU ; Eklund, Greta LU and Alvarado-Kristensson, Maria LU
- organization
- publishing date
- 2016-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biological sciences, Cell biology
- in
- Heliyon
- volume
- 2
- issue
- 9
- article number
- e00166
- publisher
- Elsevier
- external identifiers
-
- pmid:27699285
- scopus:84991329275
- ISSN
- 2405-8440
- DOI
- 10.1016/j.heliyon.2016.e00166
- language
- English
- LU publication?
- yes
- id
- 6f535b02-5150-4aa8-89c7-905e5c20dec8
- date added to LUP
- 2016-11-23 13:37:46
- date last changed
- 2024-01-19 13:45:40
@article{6f535b02-5150-4aa8-89c7-905e5c20dec8,
abstract = {{<p>The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.</p>}},
author = {{Rossello Castillo, Catalina Ana and Lindström, Lisa and Glindre, Johan and Eklund, Greta and Alvarado-Kristensson, Maria}},
issn = {{2405-8440}},
keywords = {{Biological sciences; Cell biology}},
language = {{eng}},
month = {{09}},
number = {{9}},
publisher = {{Elsevier}},
series = {{Heliyon}},
title = {{Gamma-tubulin coordinates nuclear envelope assembly around chromatin}},
url = {{http://dx.doi.org/10.1016/j.heliyon.2016.e00166}},
doi = {{10.1016/j.heliyon.2016.e00166}},
volume = {{2}},
year = {{2016}},
}