Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix
(2014) In Journal of Biomedical Optics 19(8).- Abstract
- Fourier transform infrared (FT-IR) spectroscopy was applied to characterize the extracellular matrix (ECM) of kidney tumor tissue and normal kidney tissue. Freshly resected tissue samples from 31 patients were pressed on a CaF2 substrate. FT-IR spectra obtained from ECM of tumor tissue exhibit stronger absorption bands in the spectral region from 1000 to 1200 cm−1 and around 1750 cm−1 than those obtained from normal tissue. It is likely that the spectra of ECM of kidney tumor tissue with large increases in the intensities of these
bands represent a higher concentration of fatty acids and glycerol. Amide I and amide II bands are stronger in the spectra of ECM from normal tissue, indicating a higher level of proteins. Our results suggest... (More) - Fourier transform infrared (FT-IR) spectroscopy was applied to characterize the extracellular matrix (ECM) of kidney tumor tissue and normal kidney tissue. Freshly resected tissue samples from 31 patients were pressed on a CaF2 substrate. FT-IR spectra obtained from ECM of tumor tissue exhibit stronger absorption bands in the spectral region from 1000 to 1200 cm−1 and around 1750 cm−1 than those obtained from normal tissue. It is likely that the spectra of ECM of kidney tumor tissue with large increases in the intensities of these
bands represent a higher concentration of fatty acids and glycerol. Amide I and amide II bands are stronger in the spectra of ECM from normal tissue, indicating a higher level of proteins. Our results suggest that FT-IR spectroscopy of the ECM is an innovative emerging technology for real-time intraoperative tumor diagnosis, which may improve margin clearance in renal cancer surgery. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/70e04854-3491-4962-bed7-278e0a540472
- author
- Urboniene, Vidita ; Pucetaite, Milda LU ; Jankevicius, Feliksas ; Zelvys, Arunas ; Sablinskas, Valdas and Steiner, Gerald
- publishing date
- 2014-08
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Biomedical Optics
- volume
- 19
- issue
- 8
- article number
- 087005
- publisher
- SPIE
- external identifiers
-
- scopus:84929503777
- ISSN
- 1083-3668
- DOI
- 10.1117/1.JBO.19.8.087005
- language
- English
- LU publication?
- no
- id
- 70e04854-3491-4962-bed7-278e0a540472
- date added to LUP
- 2019-03-26 10:33:30
- date last changed
- 2022-02-15 17:32:17
@article{70e04854-3491-4962-bed7-278e0a540472, abstract = {{Fourier transform infrared (FT-IR) spectroscopy was applied to characterize the extracellular matrix (ECM) of kidney tumor tissue and normal kidney tissue. Freshly resected tissue samples from 31 patients were pressed on a CaF2 substrate. FT-IR spectra obtained from ECM of tumor tissue exhibit stronger absorption bands in the spectral region from 1000 to 1200 cm−1 and around 1750 cm−1 than those obtained from normal tissue. It is likely that the spectra of ECM of kidney tumor tissue with large increases in the intensities of these<br/>bands represent a higher concentration of fatty acids and glycerol. Amide I and amide II bands are stronger in the spectra of ECM from normal tissue, indicating a higher level of proteins. Our results suggest that FT-IR spectroscopy of the ECM is an innovative emerging technology for real-time intraoperative tumor diagnosis, which may improve margin clearance in renal cancer surgery.}}, author = {{Urboniene, Vidita and Pucetaite, Milda and Jankevicius, Feliksas and Zelvys, Arunas and Sablinskas, Valdas and Steiner, Gerald}}, issn = {{1083-3668}}, language = {{eng}}, number = {{8}}, publisher = {{SPIE}}, series = {{Journal of Biomedical Optics}}, title = {{Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix}}, url = {{http://dx.doi.org/10.1117/1.JBO.19.8.087005}}, doi = {{10.1117/1.JBO.19.8.087005}}, volume = {{19}}, year = {{2014}}, }