Escherichia coli ccm in-frame mutants can produce periplasmic cytochrome b but not cytochrome c
(1997) In FEBS Letters 410(2-3).- Abstract
- Escherichia coli CcmA, CcmB and CcmC polypeptides are required for cytochrome c synthesis and are thought to constitute the subunits of an ABC-type transporter as judged from sequence data, Using a periplasmic reporter system based on Bacillus subtilis cytochrome c-550 and E. coli cytochrome b-562 we show that the synthesis of the b-type cytochrome in the periplasm is normal in E, coli ccmA and ccmC in-frame deletion mutants, Mutants deleted for ccmF or ccmG encoding a component of a putative cytochrome c-heme lyase and a membrane bound thioredoxin-like protein, respectively, have the same phenotype, The ccm mutants produce cytochrome c-550 polypeptide, but not holocytochrome c, Taken together the results demonstrate that heme can be... (More)
- Escherichia coli CcmA, CcmB and CcmC polypeptides are required for cytochrome c synthesis and are thought to constitute the subunits of an ABC-type transporter as judged from sequence data, Using a periplasmic reporter system based on Bacillus subtilis cytochrome c-550 and E. coli cytochrome b-562 we show that the synthesis of the b-type cytochrome in the periplasm is normal in E, coli ccmA and ccmC in-frame deletion mutants, Mutants deleted for ccmF or ccmG encoding a component of a putative cytochrome c-heme lyase and a membrane bound thioredoxin-like protein, respectively, have the same phenotype, The ccm mutants produce cytochrome c-550 polypeptide, but not holocytochrome c, Taken together the results demonstrate that heme can be transported to the periplasm by a ccm-independent mechanism. (C) 1997 Federation of European Biochemical Societies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7b905fe6-cfc7-48a0-99ee-8a7bfcb54b74
- author
- Throne-Holst, Mimmi LU ; Thöny-Meyer, Linda and Hederstedt, Lars LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEBS Letters
- volume
- 410
- issue
- 2-3
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:17044454865
- ISSN
- 1873-3468
- DOI
- 10.1016/S0014-5793(97)00656-X
- language
- English
- LU publication?
- yes
- id
- 7b905fe6-cfc7-48a0-99ee-8a7bfcb54b74
- date added to LUP
- 2017-07-18 09:59:43
- date last changed
- 2022-01-30 21:32:22
@article{7b905fe6-cfc7-48a0-99ee-8a7bfcb54b74, abstract = {{Escherichia coli CcmA, CcmB and CcmC polypeptides are required for cytochrome c synthesis and are thought to constitute the subunits of an ABC-type transporter as judged from sequence data, Using a periplasmic reporter system based on Bacillus subtilis cytochrome c-550 and E. coli cytochrome b-562 we show that the synthesis of the b-type cytochrome in the periplasm is normal in E, coli ccmA and ccmC in-frame deletion mutants, Mutants deleted for ccmF or ccmG encoding a component of a putative cytochrome c-heme lyase and a membrane bound thioredoxin-like protein, respectively, have the same phenotype, The ccm mutants produce cytochrome c-550 polypeptide, but not holocytochrome c, Taken together the results demonstrate that heme can be transported to the periplasm by a ccm-independent mechanism. (C) 1997 Federation of European Biochemical Societies.}}, author = {{Throne-Holst, Mimmi and Thöny-Meyer, Linda and Hederstedt, Lars}}, issn = {{1873-3468}}, language = {{eng}}, number = {{2-3}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{<em>Escherichia coli ccm</em> in-frame mutants can produce periplasmic cytochrome b but not cytochrome c}}, url = {{http://dx.doi.org/10.1016/S0014-5793(97)00656-X}}, doi = {{10.1016/S0014-5793(97)00656-X}}, volume = {{410}}, year = {{1997}}, }