Microfluidic Particle Sorting in Concentrated Erythrocyte Suspensions
Holm, Stefan H. LU ; Zhang, Zunmin ; Beech, Jason P. LU ; Gompper, Gerhard ; Fedosov, Dmitry A. and Tegenfeldt, Jonas O. LU
(2019)
In Physical Review Applied
12(1).
- Abstract
An important step in diagnostics is the isolation of specific cells and microorganisms of interest from blood. Since such bioparticles are often present at very low concentrations, throughput needs to be as high as possible. In addition, to ensure simplicity, a minimum of sample preparation is important. Therefore, sorting schemes that function for whole blood are highly desirable. Deterministic lateral displacement (DLD) devices have proven to be very precise and versatile in terms of a wide range of sorting parameters. To better understand how DLD devices perform for blood as the hematocrit increases, we carry out measurements and simulations for spherical particles in the micrometer range which move through DLD arrays for different... (More)
An important step in diagnostics is the isolation of specific cells and microorganisms of interest from blood. Since such bioparticles are often present at very low concentrations, throughput needs to be as high as possible. In addition, to ensure simplicity, a minimum of sample preparation is important. Therefore, sorting schemes that function for whole blood are highly desirable. Deterministic lateral displacement (DLD) devices have proven to be very precise and versatile in terms of a wide range of sorting parameters. To better understand how DLD devices perform for blood as the hematocrit increases, we carry out measurements and simulations for spherical particles in the micrometer range which move through DLD arrays for different flow velocities and hematocrits ranging from pure buffer to concentrated erythrocyte suspensions mimicking whole blood. We find that the separation function of the DLD array is sustained even though the blood cells introduce a shift in the trajectories and a significant dispersion for particles whose diameters are close to the critical size in the device. Simulations qualitatively replicate our experimental observations and help us identify fundamental mechanisms for the effect of hematocrit on the performance of the DLD device.
(Less)
- author
- Holm, Stefan H.
LU
; Zhang, Zunmin
; Beech, Jason P.
LU
; Gompper, Gerhard
; Fedosov, Dmitry A.
and Tegenfeldt, Jonas O.
LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physical Review Applied
- volume
- 12
- issue
- 1
- article number
- 014051
- publisher
- American Physical Society
- external identifiers
-
- scopus:85073642503
- ISSN
- 2331-7019
- DOI
- 10.1103/PhysRevApplied.12.014051
- language
- English
- LU publication?
- yes
- id
- 8284046b-64d9-4b3b-918b-bd3764f9c5e6
- date added to LUP
- 2019-11-05 09:36:28
- date last changed
- 2023-11-19 18:18:34
@article{8284046b-64d9-4b3b-918b-bd3764f9c5e6,
abstract = {{<p>An important step in diagnostics is the isolation of specific cells and microorganisms of interest from blood. Since such bioparticles are often present at very low concentrations, throughput needs to be as high as possible. In addition, to ensure simplicity, a minimum of sample preparation is important. Therefore, sorting schemes that function for whole blood are highly desirable. Deterministic lateral displacement (DLD) devices have proven to be very precise and versatile in terms of a wide range of sorting parameters. To better understand how DLD devices perform for blood as the hematocrit increases, we carry out measurements and simulations for spherical particles in the micrometer range which move through DLD arrays for different flow velocities and hematocrits ranging from pure buffer to concentrated erythrocyte suspensions mimicking whole blood. We find that the separation function of the DLD array is sustained even though the blood cells introduce a shift in the trajectories and a significant dispersion for particles whose diameters are close to the critical size in the device. Simulations qualitatively replicate our experimental observations and help us identify fundamental mechanisms for the effect of hematocrit on the performance of the DLD device.</p>}},
author = {{Holm, Stefan H. and Zhang, Zunmin and Beech, Jason P. and Gompper, Gerhard and Fedosov, Dmitry A. and Tegenfeldt, Jonas O.}},
issn = {{2331-7019}},
language = {{eng}},
number = {{1}},
publisher = {{American Physical Society}},
series = {{Physical Review Applied}},
title = {{Microfluidic Particle Sorting in Concentrated Erythrocyte Suspensions}},
url = {{http://dx.doi.org/10.1103/PhysRevApplied.12.014051}},
doi = {{10.1103/PhysRevApplied.12.014051}},
volume = {{12}},
year = {{2019}},
}