Individual aggregates of amyloid beta induce temporary calcium influx through the cell membrane of neuronal cells
(2016) In Scientific Reports 6.- Abstract
Local delivery of amyloid beta oligomers from the tip of a nanopipette, controlled over the cell surface, has been used to deliver physiological picomolar oligomer concentrations to primary astrocytes or neurons. Calcium influx was observed when as few as 2000 oligomers were delivered to the cell surface. When the dosing of oligomers was stopped the intracellular calcium returned to basal levels or below. Calcium influx was prevented by the presence in the pipette of the extracellular chaperone clusterin, which is known to selectively bind oligomers, and by the presence a specific nanobody to amyloid beta. These data are consistent with individual oligomers larger than trimers inducing calcium entry as they cross the cell membrane, a... (More)
Local delivery of amyloid beta oligomers from the tip of a nanopipette, controlled over the cell surface, has been used to deliver physiological picomolar oligomer concentrations to primary astrocytes or neurons. Calcium influx was observed when as few as 2000 oligomers were delivered to the cell surface. When the dosing of oligomers was stopped the intracellular calcium returned to basal levels or below. Calcium influx was prevented by the presence in the pipette of the extracellular chaperone clusterin, which is known to selectively bind oligomers, and by the presence a specific nanobody to amyloid beta. These data are consistent with individual oligomers larger than trimers inducing calcium entry as they cross the cell membrane, a result supported by imaging experiments in bilayers, and suggest that the initial molecular event that leads to neuronal damage does not involve any cellular receptors, in contrast to work performed at much higher oligomer concentrations.
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- author
- Drews, Anna ; Flint, Jennie ; Shivji, Nadia ; Jönsson, Peter LU ; Wirthensohn, David ; De Genst, Erwin ; Vincke, Cécile ; Muyldermans, Serge ; Dobson, Chris and Klenerman, David
- organization
- publishing date
- 2016-08-24
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 6
- article number
- 31910
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:27553885
- wos:000381831300001
- scopus:84984706229
- ISSN
- 2045-2322
- DOI
- 10.1038/srep31910
- project
- Intermolecular interactions between molecules on the surface of cells
- language
- English
- LU publication?
- yes
- id
- 8339fb1c-5aac-4df3-aed8-4631f5bfceb4
- date added to LUP
- 2016-12-02 12:58:08
- date last changed
- 2024-06-28 20:16:06
@article{8339fb1c-5aac-4df3-aed8-4631f5bfceb4,
abstract = {{<p>Local delivery of amyloid beta oligomers from the tip of a nanopipette, controlled over the cell surface, has been used to deliver physiological picomolar oligomer concentrations to primary astrocytes or neurons. Calcium influx was observed when as few as 2000 oligomers were delivered to the cell surface. When the dosing of oligomers was stopped the intracellular calcium returned to basal levels or below. Calcium influx was prevented by the presence in the pipette of the extracellular chaperone clusterin, which is known to selectively bind oligomers, and by the presence a specific nanobody to amyloid beta. These data are consistent with individual oligomers larger than trimers inducing calcium entry as they cross the cell membrane, a result supported by imaging experiments in bilayers, and suggest that the initial molecular event that leads to neuronal damage does not involve any cellular receptors, in contrast to work performed at much higher oligomer concentrations.</p>}},
author = {{Drews, Anna and Flint, Jennie and Shivji, Nadia and Jönsson, Peter and Wirthensohn, David and De Genst, Erwin and Vincke, Cécile and Muyldermans, Serge and Dobson, Chris and Klenerman, David}},
issn = {{2045-2322}},
language = {{eng}},
month = {{08}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Individual aggregates of amyloid beta induce temporary calcium influx through the cell membrane of neuronal cells}},
url = {{http://dx.doi.org/10.1038/srep31910}},
doi = {{10.1038/srep31910}},
volume = {{6}},
year = {{2016}},
}