Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
(2017) In EuPA Open Proteomics 16. p.1-11- Abstract
SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those... (More)
SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.
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- author
- Murillo, Jimmy Rodriguez ; Goto-Silva, Livia ; Sánchez, Aniel LU ; Nogueira, Fábio C.S. ; Domont, Gilberto B. and Junqueira, Magno
- organization
- publishing date
- 2017-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- iTRAQ-based proteomics, Neuronal differentiation, Phosphoproteomics, SH-SY5Y cells
- in
- EuPA Open Proteomics
- volume
- 16
- pages
- 11 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:29900121
- scopus:85023609384
- ISSN
- 2212-9685
- DOI
- 10.1016/j.euprot.2017.06.001
- language
- English
- LU publication?
- yes
- id
- 8ddd43a1-f407-4ff2-8343-799155ece914
- date added to LUP
- 2017-07-27 09:38:53
- date last changed
- 2024-04-14 15:42:27
@article{8ddd43a1-f407-4ff2-8343-799155ece914,
abstract = {{<p>SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.</p>}},
author = {{Murillo, Jimmy Rodriguez and Goto-Silva, Livia and Sánchez, Aniel and Nogueira, Fábio C.S. and Domont, Gilberto B. and Junqueira, Magno}},
issn = {{2212-9685}},
keywords = {{iTRAQ-based proteomics; Neuronal differentiation; Phosphoproteomics; SH-SY5Y cells}},
language = {{eng}},
month = {{09}},
pages = {{1--11}},
publisher = {{Elsevier}},
series = {{EuPA Open Proteomics}},
title = {{Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells}},
url = {{http://dx.doi.org/10.1016/j.euprot.2017.06.001}},
doi = {{10.1016/j.euprot.2017.06.001}},
volume = {{16}},
year = {{2017}},
}