Polymeric integrated selective enrichment target (ISET) for solid-phase-based sample preparation in MALDI-TOF MS
(2007) In Journal of Mass Spectrometry 42(11). p.1445-1452- Abstract
- d A polymer microfabricated proteomic sample preparation and MALDI MS sample presentation device, the integrated selective enrichment target (ISET), comprising an array of perforated nanovials is reported. Each perforated nanovial can be filled with selective extraction media (microbeads) for purification and concentration of protein/peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The main areas covered are the influence of the molding-process-induced surface roughness and how to address the lack of inherent conductivity in the polyetheretherketone (PEEK) material for optimal MALDI MS readout. Application of the disposable polymeric ISET devices for solid-phase extraction and phosphopeptide... (More)
- d A polymer microfabricated proteomic sample preparation and MALDI MS sample presentation device, the integrated selective enrichment target (ISET), comprising an array of perforated nanovials is reported. Each perforated nanovial can be filled with selective extraction media (microbeads) for purification and concentration of protein/peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The main areas covered are the influence of the molding-process-induced surface roughness and how to address the lack of inherent conductivity in the polyetheretherketone (PEEK) material for optimal MALDI MS readout. Application of the disposable polymeric ISET devices for solid-phase extraction and phosphopeptide capture is also demonstrated. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/969020
- author
- Ekström, Simon LU ; Wallman, Lars LU ; Helldin, Goran ; Nilsson, Johan LU ; Marko-Varga, György LU and Laurell, Thomas LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- polymeric MALDI, microfluidics, SPE, MALDI MS, solid-phase extraction, ISET, target
- in
- Journal of Mass Spectrometry
- volume
- 42
- issue
- 11
- pages
- 1445 - 1452
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000250987600007
- scopus:36148999491
- ISSN
- 1076-5174
- DOI
- 10.1002/jms.1278
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)
- id
- c67db9b5-2df2-445a-8301-1dd2786008c8 (old id 969020)
- date added to LUP
- 2016-04-01 16:01:36
- date last changed
- 2022-02-05 05:17:57
@article{c67db9b5-2df2-445a-8301-1dd2786008c8, abstract = {{d A polymer microfabricated proteomic sample preparation and MALDI MS sample presentation device, the integrated selective enrichment target (ISET), comprising an array of perforated nanovials is reported. Each perforated nanovial can be filled with selective extraction media (microbeads) for purification and concentration of protein/peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The main areas covered are the influence of the molding-process-induced surface roughness and how to address the lack of inherent conductivity in the polyetheretherketone (PEEK) material for optimal MALDI MS readout. Application of the disposable polymeric ISET devices for solid-phase extraction and phosphopeptide capture is also demonstrated.}}, author = {{Ekström, Simon and Wallman, Lars and Helldin, Goran and Nilsson, Johan and Marko-Varga, György and Laurell, Thomas}}, issn = {{1076-5174}}, keywords = {{polymeric MALDI; microfluidics; SPE; MALDI MS; solid-phase extraction; ISET; target}}, language = {{eng}}, number = {{11}}, pages = {{1445--1452}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Mass Spectrometry}}, title = {{Polymeric integrated selective enrichment target (ISET) for solid-phase-based sample preparation in MALDI-TOF MS}}, url = {{http://dx.doi.org/10.1002/jms.1278}}, doi = {{10.1002/jms.1278}}, volume = {{42}}, year = {{2007}}, }