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Purification of F.VIII:C by antigen-antibody chromatography

Ljung, R. LU orcid and Holmberg, Lars LU (1978) In Thrombosis Research 12(4). p.667-675
Abstract

Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin.

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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Thrombosis Research
volume
12
issue
4
pages
9 pages
publisher
Elsevier
external identifiers
  • pmid:663902
  • scopus:0018150726
ISSN
0049-3848
DOI
10.1016/0049-3848(78)90256-6
language
English
LU publication?
yes
id
98f17493-c0fd-48e8-83e1-0824ada050e2
date added to LUP
2016-10-26 13:52:29
date last changed
2024-01-04 14:59:24
@article{98f17493-c0fd-48e8-83e1-0824ada050e2,
  abstract     = {{<p>Purification of F.VIII:C devoid of F.VIII:Ag was achieved by antigen-antibody chromatography. The antibody used neutralized VIIIR:Ag but not VIII:C in liquid phase but extracted both VIII:Ag and VIII:C from plasma when bound to Sepharose. VIII:C was eluted with calcium-containing buffer. When plasma was used as starting material VIII:C was obtained free from VIIIR:Ag but contaminated with some other proteins. When a well-defined pure F.VIII preparation was used as starting material the VIII:C active fractions contained no immunoradiometrically detectable VIIIR:Ag, no VIIIR:RCF and no detectable protein. When stabilized with albumin VIII:C could be frozen and thawed with retained activity and could be activated with thrombin.</p>}},
  author       = {{Ljung, R. and Holmberg, Lars}},
  issn         = {{0049-3848}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{667--675}},
  publisher    = {{Elsevier}},
  series       = {{Thrombosis Research}},
  title        = {{Purification of F.VIII:C by antigen-antibody chromatography}},
  url          = {{http://dx.doi.org/10.1016/0049-3848(78)90256-6}},
  doi          = {{10.1016/0049-3848(78)90256-6}},
  volume       = {{12}},
  year         = {{1978}},
}