Purification and partial characterization of rat liver soluble catechol- O-methyltransferase
(1990) In FEBS Letters 264(1). p.95-99- Abstract
The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.
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https://lup.lub.lu.se/record/9a9cc68d-8609-4e43-9884-a599a5e42c52
- author
- Tilgmann, Carola LU and Kalkkinen, Nisse
- publishing date
- 1990-05-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Catechol-O-methyltransferase, soluble, Methylating enzyme
- in
- FEBS Letters
- volume
- 264
- issue
- 1
- pages
- 5 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:2338148
- scopus:0025355104
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(90)80774-D
- language
- English
- LU publication?
- no
- id
- 9a9cc68d-8609-4e43-9884-a599a5e42c52
- date added to LUP
- 2016-04-11 13:26:35
- date last changed
- 2024-04-04 17:46:26
@article{9a9cc68d-8609-4e43-9884-a599a5e42c52, abstract = {{<p>The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.</p>}}, author = {{Tilgmann, Carola and Kalkkinen, Nisse}}, issn = {{0014-5793}}, keywords = {{Catechol-O-methyltransferase, soluble; Methylating enzyme}}, language = {{eng}}, month = {{05}}, number = {{1}}, pages = {{95--99}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Purification and partial characterization of rat liver soluble catechol- O-methyltransferase}}, url = {{http://dx.doi.org/10.1016/0014-5793(90)80774-D}}, doi = {{10.1016/0014-5793(90)80774-D}}, volume = {{264}}, year = {{1990}}, }