Acoustofluidic harvesting of microalgae on a single chip
(2016) In Biomicrofluidics 10(3).- Abstract
We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76,... (More)
We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76, and a cell viability rate of 98.3% at a high volume rate of 500 μl/min. Our results indicate that the on-chip acoustofluidic platform is an effective tool for harvesting target microalgae from mixed populations of microalgae and other contaminants.
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- author
- Park, Jee Woong ; Kim, Soo Hyeon ; Ito, Takuro ; Fujii, Teruo ; Kim, So Youn ; Laurell, Thomas LU ; Lee, Sang Wook LU and Goda, Keisuke
- organization
- publishing date
- 2016-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomicrofluidics
- volume
- 10
- issue
- 3
- article number
- 034119
- publisher
- American Institute of Physics (AIP)
- external identifiers
-
- pmid:27462380
- wos:000379162400033
- scopus:84975452243
- ISSN
- 1932-1058
- DOI
- 10.1063/1.4954744
- language
- English
- LU publication?
- yes
- id
- a0714132-8b94-43a0-bf4b-e40d6adf0a7c
- date added to LUP
- 2017-02-02 13:53:17
- date last changed
- 2024-03-07 21:32:13
@article{a0714132-8b94-43a0-bf4b-e40d6adf0a7c,
abstract = {{<p>We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76, and a cell viability rate of 98.3% at a high volume rate of 500 μl/min. Our results indicate that the on-chip acoustofluidic platform is an effective tool for harvesting target microalgae from mixed populations of microalgae and other contaminants.</p>}},
author = {{Park, Jee Woong and Kim, Soo Hyeon and Ito, Takuro and Fujii, Teruo and Kim, So Youn and Laurell, Thomas and Lee, Sang Wook and Goda, Keisuke}},
issn = {{1932-1058}},
language = {{eng}},
month = {{05}},
number = {{3}},
publisher = {{American Institute of Physics (AIP)}},
series = {{Biomicrofluidics}},
title = {{Acoustofluidic harvesting of microalgae on a single chip}},
url = {{http://dx.doi.org/10.1063/1.4954744}},
doi = {{10.1063/1.4954744}},
volume = {{10}},
year = {{2016}},
}