Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta : Comparison to the rat liver enzyme
(1991) In Biochemical and Biophysical Research Communications 174(2). p.995-1002- Abstract
Catechol-o-methyltransferase from human placenta was purified 1400-fold by hydroxyapatite adsorption, ammonium sulfate precipitation, gel filtration, high performance anion- exchange and reversed-phase chromatography. The purified enzyme has an apparent molecular weight of 26.000, an isoelectric point of 5,3 and is activated ten-fold in the presence of 20mM cysteine. The enzyme shows primary structure homology to the corresponding rat liver soluble enzyme, based on the sequenced tryptic peptides.
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- author
- Tilgmann, Carola LU and Kalkkinen, Nisse
- publishing date
- 1991-01-31
- type
- Contribution to journal
- publication status
- published
- in
- Biochemical and Biophysical Research Communications
- volume
- 174
- issue
- 2
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0025978802
- pmid:1993083
- ISSN
- 0006-291X
- DOI
- 10.1016/0006-291X(91)91517-G
- language
- English
- LU publication?
- no
- id
- a7f41c5e-3775-467d-bf38-3d2560470f90
- date added to LUP
- 2016-04-11 13:25:49
- date last changed
- 2024-01-04 01:06:12
@article{a7f41c5e-3775-467d-bf38-3d2560470f90, abstract = {{<p>Catechol-o-methyltransferase from human placenta was purified 1400-fold by hydroxyapatite adsorption, ammonium sulfate precipitation, gel filtration, high performance anion- exchange and reversed-phase chromatography. The purified enzyme has an apparent molecular weight of 26.000, an isoelectric point of 5,3 and is activated ten-fold in the presence of 20mM cysteine. The enzyme shows primary structure homology to the corresponding rat liver soluble enzyme, based on the sequenced tryptic peptides.</p>}}, author = {{Tilgmann, Carola and Kalkkinen, Nisse}}, issn = {{0006-291X}}, language = {{eng}}, month = {{01}}, number = {{2}}, pages = {{995--1002}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta : Comparison to the rat liver enzyme}}, url = {{http://dx.doi.org/10.1016/0006-291X(91)91517-G}}, doi = {{10.1016/0006-291X(91)91517-G}}, volume = {{174}}, year = {{1991}}, }