Emi2 Is Essential for Mouse Spermatogenesis
(2017) In Cell Reports 20(3). p.697-708- Abstract
The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2—in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is... (More)
The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2—in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is associated with decreased Cdk1 activity and was partially rescued by a knockin mouse model of elevated Cdk1 activity. Additionally, we detected expression of Emi2 in spermatids and sperm, suggesting potential post-meiotic functions for Emi2.
(Less)
- author
- publishing date
- 2017-07-18
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- APC/C, Cdk1, diplotene, Emi2, knockout mice, meiosis, ovary, phosphorylation, spermatocytes, spermatogenesis
- in
- Cell Reports
- volume
- 20
- issue
- 3
- pages
- 12 pages
- publisher
- Cell Press
- external identifiers
-
- scopus:85025091691
- pmid:28723571
- ISSN
- 2211-1247
- DOI
- 10.1016/j.celrep.2017.06.033
- language
- English
- LU publication?
- no
- id
- b294e723-ae17-4d5a-9f95-ad70a13ab0e6
- date added to LUP
- 2019-09-18 10:12:05
- date last changed
- 2024-07-11 05:19:13
@article{b294e723-ae17-4d5a-9f95-ad70a13ab0e6, abstract = {{<p>The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2—in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions. We found that, besides regulating cell-cycle arrest in mouse eggs, Emi2 is essential for meiosis I progression in spermatocytes. In the absence of Emi2, spermatocytes arrest in early diplotene of prophase I. This arrest is associated with decreased Cdk1 activity and was partially rescued by a knockin mouse model of elevated Cdk1 activity. Additionally, we detected expression of Emi2 in spermatids and sperm, suggesting potential post-meiotic functions for Emi2.</p>}}, author = {{Gopinathan, Lakshmi and Szmyd, Radoslaw and Low, Diana and Diril, M. Kasim and Chang, Heng Yu and Coppola, Vincenzo and Liu, Kui and Tessarollo, Lino and Guccione, Ernesto and van Pelt, Ans M.M. and Kaldis, Philipp}}, issn = {{2211-1247}}, keywords = {{APC/C; Cdk1; diplotene; Emi2; knockout mice; meiosis; ovary; phosphorylation; spermatocytes; spermatogenesis}}, language = {{eng}}, month = {{07}}, number = {{3}}, pages = {{697--708}}, publisher = {{Cell Press}}, series = {{Cell Reports}}, title = {{Emi2 Is Essential for Mouse Spermatogenesis}}, url = {{http://dx.doi.org/10.1016/j.celrep.2017.06.033}}, doi = {{10.1016/j.celrep.2017.06.033}}, volume = {{20}}, year = {{2017}}, }