The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle
(1990) In Scandinavian Journal of Clinical & Laboratory Investigation 50(2). p.153-159- Abstract
Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The... (More)
Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p
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- author
- Clyne, N. LU ; Wibom, R. ; Havu, N. ; Hultman, E. ; Pehrsson, S. K. and Persson, B.
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- keywords
- Cobalt, Mitochondrial ATP-production, Rat myocardium, Skeletal muscle
- in
- Scandinavian Journal of Clinical & Laboratory Investigation
- volume
- 50
- issue
- 2
- pages
- 7 pages
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:2339279
- scopus:0025260789
- ISSN
- 0036-5513
- DOI
- 10.3109/00365519009089147
- language
- English
- LU publication?
- no
- id
- b7240349-08ce-472e-93ac-e5a9c6deaf3b
- date added to LUP
- 2016-08-30 22:06:47
- date last changed
- 2024-06-29 16:52:22
@article{b7240349-08ce-472e-93ac-e5a9c6deaf3b, abstract = {{<p>Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO<sub>4</sub> 7 H<sub>2</sub>O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p</p>}}, author = {{Clyne, N. and Wibom, R. and Havu, N. and Hultman, E. and Pehrsson, S. K. and Persson, B.}}, issn = {{0036-5513}}, keywords = {{Cobalt; Mitochondrial ATP-production; Rat myocardium; Skeletal muscle}}, language = {{eng}}, number = {{2}}, pages = {{153--159}}, publisher = {{Informa Healthcare}}, series = {{Scandinavian Journal of Clinical & Laboratory Investigation}}, title = {{The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle}}, url = {{http://dx.doi.org/10.3109/00365519009089147}}, doi = {{10.3109/00365519009089147}}, volume = {{50}}, year = {{1990}}, }