Acoustophoresis separation of bacteria from blood cells for rapid sepsis diagnostics
(2013) 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 2. p.1320-1322- Abstract
We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As much as 98% of the bacteria were recovered, while only 0.06 % of the red and white blood cells remained. This 1600-fold relative enrichment was achieved by adjusting the center medium density, tightly controlling the chip temperature and stabilizing the fluid flows. This is a crucial sample preparation step facilitating downstream bacteria identification for sepsis diagnostics.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d060ebb4-ab6d-4c47-965a-9188673c4049
- author
- Ohlsson, P. D. LU ; Petersson, K. LU ; Augustsson, P. LU and Laurell, T. LU
- organization
- publishing date
- 2013
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustophoresis, Bacteria, Blood, Sepsis
- host publication
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- volume
- 2
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- conference location
- Freiburg, Germany
- conference dates
- 2013-10-27 - 2013-10-31
- external identifiers
-
- scopus:84907358599
- ISBN
- 9781632666246
- language
- English
- LU publication?
- yes
- id
- d060ebb4-ab6d-4c47-965a-9188673c4049
- date added to LUP
- 2017-12-06 15:17:22
- date last changed
- 2022-02-07 17:22:00
@inproceedings{d060ebb4-ab6d-4c47-965a-9188673c4049, abstract = {{<p>We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As much as 98% of the bacteria were recovered, while only 0.06 % of the red and white blood cells remained. This 1600-fold relative enrichment was achieved by adjusting the center medium density, tightly controlling the chip temperature and stabilizing the fluid flows. This is a crucial sample preparation step facilitating downstream bacteria identification for sepsis diagnostics.</p>}}, author = {{Ohlsson, P. D. and Petersson, K. and Augustsson, P. and Laurell, T.}}, booktitle = {{17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013}}, isbn = {{9781632666246}}, keywords = {{Acoustophoresis; Bacteria; Blood; Sepsis}}, language = {{eng}}, pages = {{1320--1322}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{Acoustophoresis separation of bacteria from blood cells for rapid sepsis diagnostics}}, volume = {{2}}, year = {{2013}}, }