Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology
(2019) In RSC Advances 9(20). p.11194-11201- Abstract
Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al
2
O
3
coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a... (More)
(Less)
Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al
2
O
3
coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.
- author
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- in
- RSC Advances
- volume
- 9
- issue
- 20
- pages
- 8 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85064243221
- ISSN
- 2046-2069
- DOI
- 10.1039/c8ra05320k
- language
- English
- LU publication?
- yes
- id
- d7765a46-4e9e-4793-96be-06a693bb3203
- date added to LUP
- 2019-05-08 14:24:19
- date last changed
- 2023-11-04 12:33:57
@article{d7765a46-4e9e-4793-96be-06a693bb3203, abstract = {{<p><br> Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al <br> <sub>2</sub><br> O <br> <sub>3</sub><br> coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing. <br> </p>}}, author = {{Harberts, Jann and Zierold, Robert and Fendler, Cornelius and Koitmäe, Aune and Bayat, Parisa and Fernandez-Cuesta, Irene and Loers, Gabriele and Diercks, Björn Philipp and Fliegert, Ralf and Guse, Andreas H. and Ronning, Carsten and Otnes, Gaute and Borgström, Magnus and Blick, Robert H.}}, issn = {{2046-2069}}, language = {{eng}}, number = {{20}}, pages = {{11194--11201}}, publisher = {{Royal Society of Chemistry}}, series = {{RSC Advances}}, title = {{Culturing and patch clamping of Jurkat T cells and neurons on Al <sub>2</sub> O <sub>3</sub> coated nanowire arrays of altered morphology}}, url = {{http://dx.doi.org/10.1039/c8ra05320k}}, doi = {{10.1039/c8ra05320k}}, volume = {{9}}, year = {{2019}}, }