Intralesional EBV-DNA load as marker of prognosis for nasopharyngeal cancer
(2019) In Scientific Reports 9.- Abstract
Nasopharyngeal cancer (NPC) is associated with the Epstein-Barr virus (EBV). The clinical presentation and prognosis of NPC is well described, but not in relation to intralesional EBV-DNA load. In a retrospective design, 48 patients with NPC were examined. Patient history was re-evaluated, and diagnostic biopsies were re-examined. Furthermore, intralesional EBV-DNA was quantitated and HPV status determined. Cancer stage, disease-free survival (DFS), and overall survival (OS) were assessed. Of the 48 patients, 36 (75%) patients featured lesions that were positive for EBER (Epstein–Barr virus-encoded small RNA) and 40 (83%) were positive for EBV-DNA. Seven patients (15%) were HPV positive. The levels of EBV-DNA ranged from 0.0005 to 94617... (More)
Nasopharyngeal cancer (NPC) is associated with the Epstein-Barr virus (EBV). The clinical presentation and prognosis of NPC is well described, but not in relation to intralesional EBV-DNA load. In a retrospective design, 48 patients with NPC were examined. Patient history was re-evaluated, and diagnostic biopsies were re-examined. Furthermore, intralesional EBV-DNA was quantitated and HPV status determined. Cancer stage, disease-free survival (DFS), and overall survival (OS) were assessed. Of the 48 patients, 36 (75%) patients featured lesions that were positive for EBER (Epstein–Barr virus-encoded small RNA) and 40 (83%) were positive for EBV-DNA. Seven patients (15%) were HPV positive. The levels of EBV-DNA ranged from 0.0005 to 94617 copies/cell. An EBV-DNA load of more than 70 copies/cell was associated with a prolonged DFS for EBV-DNA positive patients treated with curative intent (p = 0.046). In conclusion, the EBV-DNA load in NPC lesions appears to vary greatly. For patients with EBV-DNA positive NPC treated with curative intent, an EBV-DNA load of more than 70 copies/cell is associated with a better outcome in terms of 7-year DFS.
(Less)
- author
- Nilsson, Johan S. LU ; Forslund, Ola LU ; Andersson, Fredrik C. ; Lindstedt, Malin LU and Greiff, Lennart LU
- organization
-
- Otorhinolaryngology (Lund)
- Division of Medical Microbiology
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Department of Immunotechnology
- Head and Neck Cancer Research Group (research group)
- Health care related infections in seriously and critically ill patients (research group)
- publishing date
- 2019-10
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 9
- article number
- 15432
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:31659192
- scopus:85074226498
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-019-51767-9
- language
- English
- LU publication?
- yes
- id
- e164b752-d26f-4506-928f-349699b66679
- date added to LUP
- 2019-11-13 13:02:47
- date last changed
- 2024-09-18 13:41:49
@article{e164b752-d26f-4506-928f-349699b66679, abstract = {{<p>Nasopharyngeal cancer (NPC) is associated with the Epstein-Barr virus (EBV). The clinical presentation and prognosis of NPC is well described, but not in relation to intralesional EBV-DNA load. In a retrospective design, 48 patients with NPC were examined. Patient history was re-evaluated, and diagnostic biopsies were re-examined. Furthermore, intralesional EBV-DNA was quantitated and HPV status determined. Cancer stage, disease-free survival (DFS), and overall survival (OS) were assessed. Of the 48 patients, 36 (75%) patients featured lesions that were positive for EBER (Epstein–Barr virus-encoded small RNA) and 40 (83%) were positive for EBV-DNA. Seven patients (15%) were HPV positive. The levels of EBV-DNA ranged from 0.0005 to 94617 copies/cell. An EBV-DNA load of more than 70 copies/cell was associated with a prolonged DFS for EBV-DNA positive patients treated with curative intent (p = 0.046). In conclusion, the EBV-DNA load in NPC lesions appears to vary greatly. For patients with EBV-DNA positive NPC treated with curative intent, an EBV-DNA load of more than 70 copies/cell is associated with a better outcome in terms of 7-year DFS.</p>}}, author = {{Nilsson, Johan S. and Forslund, Ola and Andersson, Fredrik C. and Lindstedt, Malin and Greiff, Lennart}}, issn = {{2045-2322}}, language = {{eng}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Intralesional EBV-DNA load as marker of prognosis for nasopharyngeal cancer}}, url = {{http://dx.doi.org/10.1038/s41598-019-51767-9}}, doi = {{10.1038/s41598-019-51767-9}}, volume = {{9}}, year = {{2019}}, }