Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact : A case report
(2016) In Biochemia Medica 26(2). p.279-284- Abstract
Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/e61fc36b-e931-4094-9f44-66b768544295
- author
- Anžej Doma, Sasa ; Hillarp, Andreas LU ; Pajič, Tadey ; Andoljšek, Dusan ; Černelč, Peter and Preložnik Zupan, Irena
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Autoantibody, Blood coagulation factor, Enzyme-linked immunosorbent assay, Inhibitor
- in
- Biochemia Medica
- volume
- 26
- issue
- 2
- pages
- 279 - 284
- publisher
- Biochemia Medica, Editorial Office
- external identifiers
-
- pmid:27346976
- wos:000377907400020
- scopus:84973456477
- ISSN
- 1330-0962
- DOI
- 10.11613/BM.2016.033
- language
- English
- LU publication?
- no
- id
- e61fc36b-e931-4094-9f44-66b768544295
- date added to LUP
- 2016-07-12 15:02:48
- date last changed
- 2024-03-07 09:33:55
@article{e61fc36b-e931-4094-9f44-66b768544295, abstract = {{<p>Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.</p>}}, author = {{Anžej Doma, Sasa and Hillarp, Andreas and Pajič, Tadey and Andoljšek, Dusan and Černelč, Peter and Preložnik Zupan, Irena}}, issn = {{1330-0962}}, keywords = {{Autoantibody; Blood coagulation factor; Enzyme-linked immunosorbent assay; Inhibitor}}, language = {{eng}}, number = {{2}}, pages = {{279--284}}, publisher = {{Biochemia Medica, Editorial Office}}, series = {{Biochemia Medica}}, title = {{Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact : A case report}}, url = {{http://dx.doi.org/10.11613/BM.2016.033}}, doi = {{10.11613/BM.2016.033}}, volume = {{26}}, year = {{2016}}, }