Activated human epitope-specific T cells identified by class II tetramers reside within a CD4high, proliferating subset
(2001) In International Immunology 13(6). p.799-806- Abstract
Antigen-specific T cells acquire a distinctive phenotype during activation, with characteristic acquisition of surface markers and patterns of gene expression. Early after antigen stimulation, CD4+ T lymphocytes increase their surface density of the CD4 marker, a trait which has been used to identify antigen-activated cells. The recent development of MHC tetramer technologies has greatly improved the ability to detect HLA class I-restricted T cells specific for known antigen epitopes. We have recently extended these studies to human class II-restricted CD4+ T cell responses and now describe antigen-specific T cell responses from human peripheral blood in which elevated CD4 expression levels in human T cells... (More)
Antigen-specific T cells acquire a distinctive phenotype during activation, with characteristic acquisition of surface markers and patterns of gene expression. Early after antigen stimulation, CD4+ T lymphocytes increase their surface density of the CD4 marker, a trait which has been used to identify antigen-activated cells. The recent development of MHC tetramer technologies has greatly improved the ability to detect HLA class I-restricted T cells specific for known antigen epitopes. We have recently extended these studies to human class II-restricted CD4+ T cell responses and now describe antigen-specific T cell responses from human peripheral blood in which elevated CD4 expression levels in human T cells following antigen stimulation identify the activated and proliferating subset of cells. The CD4high population is substantially enriched in epitope-specific cells identified by class II tetramer staining and almost all tetramer-positive cells are contained within the CD4high population. T cell clones derived from the tetramer-positive, CD4high population demonstrate antigen specificity and maintain tetramer staining, while the substantial number of CD4high cells which fail to stain with tetramer appear to proliferate as a result of bystander activation. Epitope-specific components of a polyclonal immune response are directly visualized and quantitated by tetramer detection, providing a direct measure of the heterogeneity of the human immune response.
(Less)
- author
- Novak, Erik J. ; Masewicz, Susan A. ; Liu, Andrew W. ; Lernmark, Åke LU ; Kwok, William W. and Nepom, Gerald T
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- keywords
- Cellular activation, FACS, HLA, MHC, T Lymphocytes
- in
- International Immunology
- volume
- 13
- issue
- 6
- pages
- 8 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:11369708
- scopus:0034992147
- ISSN
- 0953-8178
- language
- English
- LU publication?
- no
- id
- e6bef0dc-77dc-41e1-b14f-f8876149061c
- date added to LUP
- 2017-09-07 09:14:51
- date last changed
- 2024-04-14 17:34:02
@article{e6bef0dc-77dc-41e1-b14f-f8876149061c, abstract = {{<p>Antigen-specific T cells acquire a distinctive phenotype during activation, with characteristic acquisition of surface markers and patterns of gene expression. Early after antigen stimulation, CD4<sup>+</sup> T lymphocytes increase their surface density of the CD4 marker, a trait which has been used to identify antigen-activated cells. The recent development of MHC tetramer technologies has greatly improved the ability to detect HLA class I-restricted T cells specific for known antigen epitopes. We have recently extended these studies to human class II-restricted CD4<sup>+</sup> T cell responses and now describe antigen-specific T cell responses from human peripheral blood in which elevated CD4 expression levels in human T cells following antigen stimulation identify the activated and proliferating subset of cells. The CD4<sup>high</sup> population is substantially enriched in epitope-specific cells identified by class II tetramer staining and almost all tetramer-positive cells are contained within the CD4<sup>high</sup> population. T cell clones derived from the tetramer-positive, CD4<sup>high</sup> population demonstrate antigen specificity and maintain tetramer staining, while the substantial number of CD4<sup>high</sup> cells which fail to stain with tetramer appear to proliferate as a result of bystander activation. Epitope-specific components of a polyclonal immune response are directly visualized and quantitated by tetramer detection, providing a direct measure of the heterogeneity of the human immune response.</p>}}, author = {{Novak, Erik J. and Masewicz, Susan A. and Liu, Andrew W. and Lernmark, Åke and Kwok, William W. and Nepom, Gerald T}}, issn = {{0953-8178}}, keywords = {{Cellular activation; FACS; HLA; MHC; T Lymphocytes}}, language = {{eng}}, number = {{6}}, pages = {{799--806}}, publisher = {{Oxford University Press}}, series = {{International Immunology}}, title = {{Activated human epitope-specific T cells identified by class II tetramers reside within a CD4high, proliferating subset}}, volume = {{13}}, year = {{2001}}, }