Effect of impaired glucose tolerance on atherosclerotic lesion formation : An evaluation in selectively bred mice with different susceptibilities to glucose intolerance
(2013) In Atherosclerosis 231(2). p.421-426- Abstract
Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28... (More)
Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28 weeks of age). Oral glucose tolerance tests were performed during the AD-feeding period. Atherosclerotic lesion formation was quantitatively analyzed in serial aortic sinus sections by oil red O staining. Plasma lipids were measured after the AD-feeding period. Results: Glucose tolerance was impaired in SDG-P mice as compared to SDG-R mice over the 20-week AD-feeding period. No significant differences were observed in any plasma lipid measurement between the 2 mouse lines. Aortic sinus atherosclerotic lesion formation in SDG-P mice was approximately 4-fold greater than that in SDG-R mice. Conclusion: In 2 mouse lines with different susceptibilities to diet-induced glucose intolerance, IGT accelerated atherosclerotic lesion formation. These mice may therefore serve as useful invivo models for investigating the causal role of IGT in the pathogenesis of atherosclerosis.
(Less)
- author
- Asai, Akira ; Nagao, Mototsugu LU ; Kawahara, Momoyo ; Shuto, Yuki ; Sugihara, Hitoshi and Oikawa, Shinichi
- publishing date
- 2013-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animal model, Atherosclerosis, Impaired glucose tolerance, Postprandial hyperglycemia
- in
- Atherosclerosis
- volume
- 231
- issue
- 2
- pages
- 421 - 426
- publisher
- Elsevier
- external identifiers
-
- scopus:84888146994
- ISSN
- 0021-9150
- DOI
- 10.1016/j.atherosclerosis.2013.10.009
- language
- English
- LU publication?
- no
- id
- e736c470-39b6-4619-90e5-23a27c2979a8
- date added to LUP
- 2017-08-23 20:03:06
- date last changed
- 2022-04-09 18:25:33
@article{e736c470-39b6-4619-90e5-23a27c2979a8, abstract = {{<p>Objective: Impaired glucose tolerance (IGT) is an independent risk factor for atherosclerotic cardiovascular disease. However, due to the lack of appropriate animal models, the underlying mechanisms for IGT-induced atherosclerosis remain to be elucidated invivo. We recently used selective breeding to establish 2 mouse lines with distinctively different susceptibilities to diet-induced glucose intolerance, designated selectively bred diet-induced glucose intolerance-resistant (SDG-R) and SDG-prone (SDG-P), respectively. Here, we assessed atherosclerotic lesion formation in these mice. Methods: Female SDG-R and SDG-P mice were fed an atherogenic diet (AD; 1.25% cholesterol, 0.5% sodium cholate, and 36% energy as fat) for 20 weeks (8-28 weeks of age). Oral glucose tolerance tests were performed during the AD-feeding period. Atherosclerotic lesion formation was quantitatively analyzed in serial aortic sinus sections by oil red O staining. Plasma lipids were measured after the AD-feeding period. Results: Glucose tolerance was impaired in SDG-P mice as compared to SDG-R mice over the 20-week AD-feeding period. No significant differences were observed in any plasma lipid measurement between the 2 mouse lines. Aortic sinus atherosclerotic lesion formation in SDG-P mice was approximately 4-fold greater than that in SDG-R mice. Conclusion: In 2 mouse lines with different susceptibilities to diet-induced glucose intolerance, IGT accelerated atherosclerotic lesion formation. These mice may therefore serve as useful invivo models for investigating the causal role of IGT in the pathogenesis of atherosclerosis.</p>}}, author = {{Asai, Akira and Nagao, Mototsugu and Kawahara, Momoyo and Shuto, Yuki and Sugihara, Hitoshi and Oikawa, Shinichi}}, issn = {{0021-9150}}, keywords = {{Animal model; Atherosclerosis; Impaired glucose tolerance; Postprandial hyperglycemia}}, language = {{eng}}, number = {{2}}, pages = {{421--426}}, publisher = {{Elsevier}}, series = {{Atherosclerosis}}, title = {{Effect of impaired glucose tolerance on atherosclerotic lesion formation : An evaluation in selectively bred mice with different susceptibilities to glucose intolerance}}, url = {{http://dx.doi.org/10.1016/j.atherosclerosis.2013.10.009}}, doi = {{10.1016/j.atherosclerosis.2013.10.009}}, volume = {{231}}, year = {{2013}}, }