Effects of naloxone on c-jun/AP-1 in MET-enkephalin- and FMRFamide-immunoreactive neurons of a gastropod snail
(1999) In Acta Biologica Hungarica 50(1-3). p.43-54- Abstract
- 1. Opioid- and FMRFamide (FMRFa)-ergic systems are believed to play antagonistic behavioral roles in both higher and lower animals. In our previous experiments on a snail, behavioral choice has been demonstrated to be dependent on a balance between FMRFa and enkephalins [7]. Here, we examined if the disturbance of the balance causes changes in the activity of both systems. Opiate receptor blocker naloxone was applied and its effect on c-jun expression of met-enkephalin (MEnk)- and FMRFa-ergic neurons was examined immunocytochemically in terrestrial gastropod snail Cepaea nemoralis. 2. In control, untreated snails, central neurons with c-jun/AP-1-like-immunoreactivity were found to occur. These included MEnk-, FMRFa- and 5HT-immunoreactive... (More)
- 1. Opioid- and FMRFamide (FMRFa)-ergic systems are believed to play antagonistic behavioral roles in both higher and lower animals. In our previous experiments on a snail, behavioral choice has been demonstrated to be dependent on a balance between FMRFa and enkephalins [7]. Here, we examined if the disturbance of the balance causes changes in the activity of both systems. Opiate receptor blocker naloxone was applied and its effect on c-jun expression of met-enkephalin (MEnk)- and FMRFa-ergic neurons was examined immunocytochemically in terrestrial gastropod snail Cepaea nemoralis. 2. In control, untreated snails, central neurons with c-jun/AP-1-like-immunoreactivity were found to occur. These included MEnk-, FMRFa- and 5HT-immunoreactive (-ir) neurons, as was revealed by double-labelling. 3. After treatment with naloxone for 4 h, the following changes were observed: (i) increase in the number of MEnk-ir neurons; increase in the number of neurons showing c-jun/AP-1 and MEnk double-labeling; (ii) disappearance of c-jun/AP-1-immunoreactivity from some FMRFa-ir neurons. 4. It is suggested that immediate early genes are involved in the mechanisms responsible for the reciprocal regulation of the opioid and antiopioid neuropeptide systems. (Less)
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https://lup.lub.lu.se/record/ed31e956-ed17-4182-b6be-ca3e742c6f6a
- author
- Dyakonova, V ; Elofsson, Rolf LU ; Carlberg, M and Sakharov, D A
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Biologica Hungarica
- volume
- 50
- issue
- 1-3
- pages
- 43 - 54
- publisher
- Akademiai Kiado
- external identifiers
-
- scopus:0032730282
- pmid:10574428
- ISSN
- 0236-5383
- language
- English
- LU publication?
- yes
- id
- ed31e956-ed17-4182-b6be-ca3e742c6f6a
- date added to LUP
- 2016-10-31 21:17:18
- date last changed
- 2022-01-30 07:07:08
@article{ed31e956-ed17-4182-b6be-ca3e742c6f6a,
abstract = {{1. Opioid- and FMRFamide (FMRFa)-ergic systems are believed to play antagonistic behavioral roles in both higher and lower animals. In our previous experiments on a snail, behavioral choice has been demonstrated to be dependent on a balance between FMRFa and enkephalins [7]. Here, we examined if the disturbance of the balance causes changes in the activity of both systems. Opiate receptor blocker naloxone was applied and its effect on c-jun expression of met-enkephalin (MEnk)- and FMRFa-ergic neurons was examined immunocytochemically in terrestrial gastropod snail Cepaea nemoralis. 2. In control, untreated snails, central neurons with c-jun/AP-1-like-immunoreactivity were found to occur. These included MEnk-, FMRFa- and 5HT-immunoreactive (-ir) neurons, as was revealed by double-labelling. 3. After treatment with naloxone for 4 h, the following changes were observed: (i) increase in the number of MEnk-ir neurons; increase in the number of neurons showing c-jun/AP-1 and MEnk double-labeling; (ii) disappearance of c-jun/AP-1-immunoreactivity from some FMRFa-ir neurons. 4. It is suggested that immediate early genes are involved in the mechanisms responsible for the reciprocal regulation of the opioid and antiopioid neuropeptide systems.}},
author = {{Dyakonova, V and Elofsson, Rolf and Carlberg, M and Sakharov, D A}},
issn = {{0236-5383}},
language = {{eng}},
number = {{1-3}},
pages = {{43--54}},
publisher = {{Akademiai Kiado}},
series = {{Acta Biologica Hungarica}},
title = {{Effects of naloxone on c-jun/AP-1 in MET-enkephalin- and FMRFamide-immunoreactive neurons of a gastropod snail}},
volume = {{50}},
year = {{1999}},
}