Comparison of Three Different TTF-1 Clones in Resected Primary Lung Cancer and Epithelial Pulmonary Metastases
(2018) In American Journal of Clinical Pathology 150(6). p.533-544- Abstract
Objectives: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.
Methods: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.
Results: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and... (More)
Objectives: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.
Methods: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.
Results: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas.
Conclusions: TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.
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- author
- Vidarsdottir, Halla LU ; Tran, Lena ; Nodin, Björn LU ; Jirström, Karin LU ; Planck, Maria LU ; Mattsson, Johanna S.M. ; Botling, Johan ; Micke, Patrick ; Jönsson, Per LU and Brunnström, Hans LU
- organization
-
- Improved diagnostics and prognostics of lung cancer and metastases to the lungs (research group)
- Personalized Pathology & Cancer Therapy (research group)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Research Group Lung Cancer (research group)
- Thoracic Surgery
- publishing date
- 2018
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Clinical Pathology
- volume
- 150
- issue
- 6
- pages
- 12 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:85055604246
- pmid:30169783
- ISSN
- 1943-7722
- DOI
- 10.1093/ajcp/aqy083
- project
- Histopathological and molecular diagnostics of lung cancer
- language
- English
- LU publication?
- yes
- id
- ee7749c8-23a1-4a20-92ec-5fe1a0c89c14
- date added to LUP
- 2018-11-15 12:01:53
- date last changed
- 2024-09-03 06:20:28
@article{ee7749c8-23a1-4a20-92ec-5fe1a0c89c14, abstract = {{<p>Objectives: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.</p><p>Methods: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.</p><p>Results: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas.</p><p>Conclusions: TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.</p>}}, author = {{Vidarsdottir, Halla and Tran, Lena and Nodin, Björn and Jirström, Karin and Planck, Maria and Mattsson, Johanna S.M. and Botling, Johan and Micke, Patrick and Jönsson, Per and Brunnström, Hans}}, issn = {{1943-7722}}, language = {{eng}}, number = {{6}}, pages = {{533--544}}, publisher = {{Oxford University Press}}, series = {{American Journal of Clinical Pathology}}, title = {{Comparison of Three Different TTF-1 Clones in Resected Primary Lung Cancer and Epithelial Pulmonary Metastases}}, url = {{http://dx.doi.org/10.1093/ajcp/aqy083}}, doi = {{10.1093/ajcp/aqy083}}, volume = {{150}}, year = {{2018}}, }