Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status
Rask, Lene ; Balslev, Eva ; Søkilde, Rolf LU
; Høgdall, Estrid
; Flyger, Henrik
; Eriksen, Jens
and Litman, Thomas
(2014)
In Cellular Oncology
37(3).
p.27-215
- Abstract
PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.
METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative... (More)
PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.
METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry.
RESULTS: miR-486-5p and miR-139-5p were found to be down-regulated in patients with lymph node metastases, whereas miR-21 was found to be up-regulated in patients with a positive lymph node status. miR-21 expression levels were found to significantly correlate with tumour size (r = 0.403, p = 0.009; Spearman's rank), whereas no relation was found between miR-21 and PTEN expression levels (Kruskal-Wallis test).
CONCLUSION: Down-regulation of miR-486-5p and miR-139-5p, in conjunction with up-regulation of miR-21, may represent a useful signature for the identification of high-risk breast cancer patients.
(Less)
- author
- Rask, Lene
; Balslev, Eva
; Søkilde, Rolf
LU
; Høgdall, Estrid
; Flyger, Henrik
; Eriksen, Jens
and Litman, Thomas
- publishing date
- 2014-06
- type
- Contribution to journal
- publication status
- published
- keywords
- Aged, Blotting, Western, Breast Neoplasms, Down-Regulation, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Lymphatic Metastasis, MicroRNAs, Middle Aged, Oligonucleotide Array Sequence Analysis, PTEN Phosphohydrolase, Reverse Transcriptase Polymerase Chain Reaction, Risk Factors, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't
- in
- Cellular Oncology
- volume
- 37
- issue
- 3
- pages
- 13 pages
- publisher
- Springer
- external identifiers
-
- pmid:25027758
- scopus:84904756218
- ISSN
- 2211-3428
- DOI
- 10.1007/s13402-014-0176-6
- language
- English
- LU publication?
- no
- id
- effd1273-6f21-4605-ae6f-8500fd91c938
- date added to LUP
- 2017-09-01 14:29:01
- date last changed
- 2024-03-31 15:59:12
@article{effd1273-6f21-4605-ae6f-8500fd91c938,
abstract = {{<p>PURPOSE: Therapeutic decisions in breast cancer are increasingly guided by prognostic and predictive biomarkers. Non-protein-coding microRNAs (miRNAs) have recently been found to be deregulated in breast cancers and, in addition, to be correlated with several clinico-pathological features. One of the most consistently up-regulated miRNAs is miR-21. Here, we specifically searched for differentially expressed miRNAs in high-risk breast cancer patients as compared to low-risk breast cancer patients. In the same patients, we also compared miR-21 expression with the expression of its presumed target PTEN.</p><p>METHODS: Both microarray and RT-qPCR techniques were used to assess miRNA expression levels in lymph node-positive and -negative human invasive ductal carcinoma tissues. Simultaneously, PTEN protein expression levels were assessed using immunohistochemistry.</p><p>RESULTS: miR-486-5p and miR-139-5p were found to be down-regulated in patients with lymph node metastases, whereas miR-21 was found to be up-regulated in patients with a positive lymph node status. miR-21 expression levels were found to significantly correlate with tumour size (r = 0.403, p = 0.009; Spearman's rank), whereas no relation was found between miR-21 and PTEN expression levels (Kruskal-Wallis test).</p><p>CONCLUSION: Down-regulation of miR-486-5p and miR-139-5p, in conjunction with up-regulation of miR-21, may represent a useful signature for the identification of high-risk breast cancer patients.</p>}},
author = {{Rask, Lene and Balslev, Eva and Søkilde, Rolf and Høgdall, Estrid and Flyger, Henrik and Eriksen, Jens and Litman, Thomas}},
issn = {{2211-3428}},
keywords = {{Aged; Blotting, Western; Breast Neoplasms; Down-Regulation; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Lymphatic Metastasis; MicroRNAs; Middle Aged; Oligonucleotide Array Sequence Analysis; PTEN Phosphohydrolase; Reverse Transcriptase Polymerase Chain Reaction; Risk Factors; Up-Regulation; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{3}},
pages = {{27--215}},
publisher = {{Springer}},
series = {{Cellular Oncology}},
title = {{Differential expression of miR-139, miR-486 and miR-21 in breast cancer patients sub-classified according to lymph node status}},
url = {{http://dx.doi.org/10.1007/s13402-014-0176-6}},
doi = {{10.1007/s13402-014-0176-6}},
volume = {{37}},
year = {{2014}},
}