Presence of vitronectin and activated complement factor C9 on ventriculoperitoneal shunts and temporary ventricular drainage catheters
Lundberg, Fredrik LU ; Li, Dai Qing ; Falkenback, Dan ; Lea, Tor ; Siesjö, Peter LU
; Söderström, Sven
; Kudryk, Bohdan J.
; Tegenfeldt, Jonas O.
LU
; Nomura, Sadahiro
and Ljungh, Åsa
(1999)
In Journal of Neurosurgery
90(1).
p.101-108
- Abstract
Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface,... (More)
Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface, immunoblotting of catheter eluates, and scanning force microscopy of immunogold bound to the catheter surface. The immunoblot showed that vitronectin was adsorbed in its native form and that fibronectin was degraded into small fragments. Furthermore, the study demonstrated that the level of vitronectin in CSF increased in patients with an impaired CSF-blood barrier. To study complement activation, an antibody that recognizes the neoepitope of activated complement factor C9 was used. The presence of activated complement factor C9 was shown on both temporary catheters and shunts. Conclusions. Activation of complement close to the surface of an inserted catheter could contribute to the pathogenesis of CSF shunt infection.
(Less)
- author
- Lundberg, Fredrik
LU
; Li, Dai Qing
; Falkenback, Dan
; Lea, Tor
; Siesjö, Peter
LU
; Söderström, Sven
; Kudryk, Bohdan J.
; Tegenfeldt, Jonas O.
LU
; Nomura, Sadahiro
and Ljungh, Åsa
- organization
- publishing date
- 1999-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Activated complement C9, Fibrinogen, Immunogold, Scanning force microscopy, Ventricular catheter, Vitronectin
- in
- Journal of Neurosurgery
- volume
- 90
- issue
- 1
- pages
- 8 pages
- publisher
- American Association of Neurosurgeons
- external identifiers
-
- pmid:10413162
- scopus:0032948821
- ISSN
- 0022-3085
- DOI
- 10.3171/jns.1999.90.1.0101
- language
- English
- LU publication?
- yes
- id
- f7ce07da-6ae2-48a6-b54b-b945e85e5c25
- date added to LUP
- 2018-10-20 10:51:39
- date last changed
- 2024-01-15 04:41:13
@article{f7ce07da-6ae2-48a6-b54b-b945e85e5c25,
abstract = {{<p>Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface, immunoblotting of catheter eluates, and scanning force microscopy of immunogold bound to the catheter surface. The immunoblot showed that vitronectin was adsorbed in its native form and that fibronectin was degraded into small fragments. Furthermore, the study demonstrated that the level of vitronectin in CSF increased in patients with an impaired CSF-blood barrier. To study complement activation, an antibody that recognizes the neoepitope of activated complement factor C9 was used. The presence of activated complement factor C9 was shown on both temporary catheters and shunts. Conclusions. Activation of complement close to the surface of an inserted catheter could contribute to the pathogenesis of CSF shunt infection.</p>}},
author = {{Lundberg, Fredrik and Li, Dai Qing and Falkenback, Dan and Lea, Tor and Siesjö, Peter and Söderström, Sven and Kudryk, Bohdan J. and Tegenfeldt, Jonas O. and Nomura, Sadahiro and Ljungh, Åsa}},
issn = {{0022-3085}},
keywords = {{Activated complement C9; Fibrinogen; Immunogold; Scanning force microscopy; Ventricular catheter; Vitronectin}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{101--108}},
publisher = {{American Association of Neurosurgeons}},
series = {{Journal of Neurosurgery}},
title = {{Presence of vitronectin and activated complement factor C9 on ventriculoperitoneal shunts and temporary ventricular drainage catheters}},
url = {{http://dx.doi.org/10.3171/jns.1999.90.1.0101}},
doi = {{10.3171/jns.1999.90.1.0101}},
volume = {{90}},
year = {{1999}},
}