Proteomic study of non-typable Haemophilus influenzae
(2002) In Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 782(1-2). p.26-219- Abstract
Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain.... (More)
Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.
(Less)
- author
- Thorén, Kajsa ; Gustafsson, Elisabet ; Clevnert, Annica ; Larsson, Thomas ; Bergström, Jörgen and Nilsson, Carol L LU
- publishing date
- 2002-12-25
- type
- Contribution to journal
- publication status
- published
- keywords
- Bacterial Proteins, Electrophoresis, Gel, Two-Dimensional, Haemophilus influenzae, Mass Spectrometry, Proteomics, Journal Article, Research Support, Non-U.S. Gov't
- in
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
- volume
- 782
- issue
- 1-2
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0037176204
- pmid:12458008
- ISSN
- 1570-0232
- language
- English
- LU publication?
- no
- id
- fc3e4d3f-eec5-4f6b-8f7e-bed105dc5dde
- date added to LUP
- 2017-05-16 10:39:30
- date last changed
- 2024-01-13 21:03:45
@article{fc3e4d3f-eec5-4f6b-8f7e-bed105dc5dde,
abstract = {{<p>Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.</p>}},
author = {{Thorén, Kajsa and Gustafsson, Elisabet and Clevnert, Annica and Larsson, Thomas and Bergström, Jörgen and Nilsson, Carol L}},
issn = {{1570-0232}},
keywords = {{Bacterial Proteins; Electrophoresis, Gel, Two-Dimensional; Haemophilus influenzae; Mass Spectrometry; Proteomics; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{12}},
number = {{1-2}},
pages = {{26--219}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences}},
title = {{Proteomic study of non-typable Haemophilus influenzae}},
volume = {{782}},
year = {{2002}},
}