14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics
(2007) In Journal of Proteome Research 6(9). p.3491-3500- Abstract
Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass... (More)
Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.
(Less)
- author
- Sihlbom, Carina ; Wilhelmsson, Ulrika ; Li, Lizhen ; Nilsson, Carol L LU and Pekny, Milos
- publishing date
- 2007-09
- type
- Contribution to journal
- publication status
- published
- keywords
- 14-3-3 Proteins, Animals, Astrocytes, Cerebral Cortex, Electrophoresis, Gel, Two-Dimensional, Entorhinal Cortex, Glial Fibrillary Acidic Protein, Hippocampus, Mass Spectrometry, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neurodegenerative Diseases, Proteomics, Vimentin, Journal Article, Research Support, Non-U.S. Gov't
- in
- Journal of Proteome Research
- volume
- 6
- issue
- 9
- pages
- 10 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:17663576
- scopus:34948836783
- ISSN
- 1535-3893
- DOI
- 10.1021/pr070108e
- language
- English
- LU publication?
- no
- id
- ff8e3c89-5e64-401a-98ef-f8ebaedc1697
- date added to LUP
- 2017-05-16 10:33:35
- date last changed
- 2024-01-13 21:00:52
@article{ff8e3c89-5e64-401a-98ef-f8ebaedc1697, abstract = {{<p>Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.</p>}}, author = {{Sihlbom, Carina and Wilhelmsson, Ulrika and Li, Lizhen and Nilsson, Carol L and Pekny, Milos}}, issn = {{1535-3893}}, keywords = {{14-3-3 Proteins; Animals; Astrocytes; Cerebral Cortex; Electrophoresis, Gel, Two-Dimensional; Entorhinal Cortex; Glial Fibrillary Acidic Protein; Hippocampus; Mass Spectrometry; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neurodegenerative Diseases; Proteomics; Vimentin; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, number = {{9}}, pages = {{3491--3500}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics}}, url = {{http://dx.doi.org/10.1021/pr070108e}}, doi = {{10.1021/pr070108e}}, volume = {{6}}, year = {{2007}}, }