Purification and partial characterization of rat liver soluble catechol- O-methyltransferase
Tilgmann, Carola LU
and Kalkkinen, Nisse
(1990)
In FEBS Letters
264(1).
p.95-99
- Abstract
The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/9a9cc68d-8609-4e43-9884-a599a5e42c52
- author
- Tilgmann, Carola
LU
and Kalkkinen, Nisse
- publishing date
- 1990-05-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Catechol-O-methyltransferase, soluble, Methylating enzyme
- in
- FEBS Letters
- volume
- 264
- issue
- 1
- pages
- 5 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:2338148
- scopus:0025355104
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(90)80774-D
- language
- English
- LU publication?
- no
- id
- 9a9cc68d-8609-4e43-9884-a599a5e42c52
- date added to LUP
- 2016-04-11 13:26:35
- date last changed
- 2024-04-04 17:46:26
@article{9a9cc68d-8609-4e43-9884-a599a5e42c52,
abstract = {{<p>The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.</p>}},
author = {{Tilgmann, Carola and Kalkkinen, Nisse}},
issn = {{0014-5793}},
keywords = {{Catechol-O-methyltransferase, soluble; Methylating enzyme}},
language = {{eng}},
month = {{05}},
number = {{1}},
pages = {{95--99}},
publisher = {{Wiley-Blackwell}},
series = {{FEBS Letters}},
title = {{Purification and partial characterization of rat liver soluble catechol- O-methyltransferase}},
url = {{http://dx.doi.org/10.1016/0014-5793(90)80774-D}},
doi = {{10.1016/0014-5793(90)80774-D}},
volume = {{264}},
year = {{1990}},
}