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Increased cyclic AMP in in vitro regenerating frog sciatic nerves inhibits Schwann cell proliferation bur has no effect on axonal outgrowth

Ekström, Per LU (1995) In Journal of Neuroscience Research 42(1). p.54-62
Abstract

In the present study the role of cAMP for axonal outgrowth and Schwann cell proliferation was studying using the cultured frog sciatic nerve. An intrinsic rise in nerve injury, both in vitro and in vivo. Treatment with 0.1‐1.0μM forskolin, an activator of the cAMP‐generating enzyme adenylyl cyclase, increased the cAMP content up to 13‐fold, but was yet without effect on axonal outgrowth during an 8‐day culturing period. HA‐1004, an inhibitor of cAMP‐dependent protein kinase, also lacked effect on the regeneraton. In contrast, the proliferation of Schwann cells, measured as [3H]thymidine incorporation, was inhibited to about 70% of control by forskolin, whereas HA‐1004 stimulated proliferation to approximately 130% of control. The... (More)

In the present study the role of cAMP for axonal outgrowth and Schwann cell proliferation was studying using the cultured frog sciatic nerve. An intrinsic rise in nerve injury, both in vitro and in vivo. Treatment with 0.1‐1.0μM forskolin, an activator of the cAMP‐generating enzyme adenylyl cyclase, increased the cAMP content up to 13‐fold, but was yet without effect on axonal outgrowth during an 8‐day culturing period. HA‐1004, an inhibitor of cAMP‐dependent protein kinase, also lacked effect on the regeneraton. In contrast, the proliferation of Schwann cells, measured as [3H]thymidine incorporation, was inhibited to about 70% of control by forskolin, whereas HA‐1004 stimulated proliferation to approximately 130% of control. The results suggest that cAMP is involved in the injury‐induced proliferation of Schwann cells of an adult peripheral nerve but that it lacks a central role in the regeneration of sensory axons of such nerves. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adenylyl cyclase, amphibian, forskolin, HA‐1004, peripheral nerve
in
Journal of Neuroscience Research
volume
42
issue
1
pages
9 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0029146612
ISSN
0360-4012
DOI
10.1002/jnr.490420107
language
English
LU publication?
yes
id
c3d7135c-4415-4f37-ab9b-29a2dcd5ddc1
date added to LUP
2016-12-07 14:50:14
date last changed
2017-01-01 08:42:31
@article{c3d7135c-4415-4f37-ab9b-29a2dcd5ddc1,
  abstract     = {<p>In the present study the role of cAMP for axonal outgrowth and Schwann cell proliferation was studying using the cultured frog sciatic nerve. An intrinsic rise in nerve injury, both in vitro and in vivo. Treatment with 0.1‐1.0μM forskolin, an activator of the cAMP‐generating enzyme adenylyl cyclase, increased the cAMP content up to 13‐fold, but was yet without effect on axonal outgrowth during an 8‐day culturing period. HA‐1004, an inhibitor of cAMP‐dependent protein kinase, also lacked effect on the regeneraton. In contrast, the proliferation of Schwann cells, measured as [3H]thymidine incorporation, was inhibited to about 70% of control by forskolin, whereas HA‐1004 stimulated proliferation to approximately 130% of control. The results suggest that cAMP is involved in the injury‐induced proliferation of Schwann cells of an adult peripheral nerve but that it lacks a central role in the regeneration of sensory axons of such nerves. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.</p>},
  author       = {Ekström, Per},
  issn         = {0360-4012},
  keyword      = {adenylyl cyclase,amphibian,forskolin,HA‐1004,peripheral nerve},
  language     = {eng},
  number       = {1},
  pages        = {54--62},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Increased cyclic AMP in in vitro regenerating frog sciatic nerves inhibits Schwann cell proliferation bur has no effect on axonal outgrowth},
  url          = {http://dx.doi.org/10.1002/jnr.490420107},
  volume       = {42},
  year         = {1995},
}