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Carrier detection in hemophilia A : a cooperative international study. II. The efficacy of a universal discriminant

Green, P P; Mannucci, P M; Briët, E; Ljung, R LU ; Kasper, C K; Essien, E M; Chediak, J; Rizza, C R and Graham, J B (1986) In Blood 67(6). p.7-1560
Abstract

Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratories and is nearly as effective as laboratory-specific discriminants; that inclusion of age and ABO blood type data improved the efficacy of these discriminants; that substitution of alternative assays for F.VIII and VWF:Ag did not generally improve the efficacy of the discriminants over that obtained using the bioassay for F.VIII:C and Laurell's... (More)

Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratories and is nearly as effective as laboratory-specific discriminants; that inclusion of age and ABO blood type data improved the efficacy of these discriminants; that substitution of alternative assays for F.VIII and VWF:Ag did not generally improve the efficacy of the discriminants over that obtained using the bioassay for F.VIII:C and Laurell's immunoassay for VWF:Ag; that linear discriminants were far more effective than discriminants based on the F.VIII:C/VWF:Ag ratio. A step-wise procedure is given which any laboratory may follow in using the universal discriminant for carrier detection.

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publication status
published
subject
keywords
ABO Blood-Group System, Adult, Antigens, Enzyme-Linked Immunosorbent Assay, Factor VIII, Female, Hemophilia A, Heterozygote Detection, Humans, Immunoenzyme Techniques, International Cooperation, Phenotype, von Willebrand Factor, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
in
Blood
volume
67
issue
6
pages
7 - 1560
publisher
American Society of Hematology
external identifiers
  • Scopus:0022453895
ISSN
0006-4971
language
English
LU publication?
yes
id
05fba024-738c-432f-9247-ee00cb4edc69
date added to LUP
2016-10-26 14:01:21
date last changed
2016-11-27 04:43:41
@misc{05fba024-738c-432f-9247-ee00cb4edc69,
  abstract     = {<p>Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratories and is nearly as effective as laboratory-specific discriminants; that inclusion of age and ABO blood type data improved the efficacy of these discriminants; that substitution of alternative assays for F.VIII and VWF:Ag did not generally improve the efficacy of the discriminants over that obtained using the bioassay for F.VIII:C and Laurell's immunoassay for VWF:Ag; that linear discriminants were far more effective than discriminants based on the F.VIII:C/VWF:Ag ratio. A step-wise procedure is given which any laboratory may follow in using the universal discriminant for carrier detection.</p>},
  author       = {Green, P P and Mannucci, P M and Briët, E and Ljung, R and Kasper, C K and Essien, E M and Chediak, J and Rizza, C R and Graham, J B},
  issn         = {0006-4971},
  keyword      = {ABO Blood-Group System,Adult,Antigens,Enzyme-Linked Immunosorbent Assay,Factor VIII,Female,Hemophilia A,Heterozygote Detection,Humans,Immunoenzyme Techniques,International Cooperation,Phenotype,von Willebrand Factor,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, P.H.S.},
  language     = {eng},
  number       = {6},
  pages        = {7--1560},
  publisher    = {ARRAY(0xd623078)},
  series       = {Blood},
  title        = {Carrier detection in hemophilia A : a cooperative international study. II. The efficacy of a universal discriminant},
  volume       = {67},
  year         = {1986},
}