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Immunohistochemical analysis of cannabinoid receptor 1 expression in steatotic rat livers

Zduniak, Krzysztof; Ziółkowski, Piotr; Regnell, Pontus; Tollet-Egnell, Petra; Åkesson, Lina LU and Cooper, Martin E. (2016) In Experimental and Therapeutic Medicine 11(4). p.1227-1230
Abstract

The primary aim of the present study was to determine the expression levels of cannabinoid receptor type 1 (CB1) in steatotic rat livers. The secondary aim was to clarify whether steatosis and inflammation are more marked in areas with increased CB1 overexpression. For ethical and economic reasons, the present study investigated tissue from archived liver blocks, which were obtained from 38 rats that had been euthanized during the course of previous research at the Karolinska Institute of the Karolinska University Hospital (Stockholm, Sweden) and Lund University (Malmö, Sweden). Liver tissue fixed in formalin and embedded in paraffin was used that had been sourced from 36 male Sprague Dawley rats (age, 7 weeks) and 2 rats (age, 180... (More)

The primary aim of the present study was to determine the expression levels of cannabinoid receptor type 1 (CB1) in steatotic rat livers. The secondary aim was to clarify whether steatosis and inflammation are more marked in areas with increased CB1 overexpression. For ethical and economic reasons, the present study investigated tissue from archived liver blocks, which were obtained from 38 rats that had been euthanized during the course of previous research at the Karolinska Institute of the Karolinska University Hospital (Stockholm, Sweden) and Lund University (Malmö, Sweden). Liver tissue fixed in formalin and embedded in paraffin was used that had been sourced from 36 male Sprague Dawley rats (age, 7 weeks) and 2 rats (age, 180 days) lacking normal leptin receptors. The rat liver tissue was stained with antibodies against CB1 and counterstained with hematoxylin. The expression of CB1 and the number of cells overexpressing CB1 were determined. Steatosis was scored according to the Dixon scoring system. CB1 overexpression and steatosis were detected in hepatocytes from all 38 livers sampled. The expression of CB1 was more marked in hepatocytes localized next to portal triads. Near the central veins, the expression was significantly weaker. Steatosis was more marked in areas of increased CB1 overexpres with publications reporting that CB1 in hepatocytes increases lipogenesis and contributes to inflammation.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cannabinoid receptor 1, Fatty liver, Immunohistochemistry, Rats
in
Experimental and Therapeutic Medicine
volume
11
issue
4
pages
4 pages
publisher
Spandidos Publications Ltd
external identifiers
  • Scopus:84958778487
ISSN
1792-0981
DOI
10.3892/etm.2016.3036
language
English
LU publication?
yes
id
0970f3b8-b1fa-4ab7-a331-a367e81ec720
date added to LUP
2016-07-25 14:24:59
date last changed
2016-11-14 09:42:58
@misc{0970f3b8-b1fa-4ab7-a331-a367e81ec720,
  abstract     = {<p>The primary aim of the present study was to determine the expression levels of cannabinoid receptor type 1 (CB1) in steatotic rat livers. The secondary aim was to clarify whether steatosis and inflammation are more marked in areas with increased CB1 overexpression. For ethical and economic reasons, the present study investigated tissue from archived liver blocks, which were obtained from 38 rats that had been euthanized during the course of previous research at the Karolinska Institute of the Karolinska University Hospital (Stockholm, Sweden) and Lund University (Malmö, Sweden). Liver tissue fixed in formalin and embedded in paraffin was used that had been sourced from 36 male Sprague Dawley rats (age, 7 weeks) and 2 rats (age, 180 days) lacking normal leptin receptors. The rat liver tissue was stained with antibodies against CB1 and counterstained with hematoxylin. The expression of CB1 and the number of cells overexpressing CB1 were determined. Steatosis was scored according to the Dixon scoring system. CB1 overexpression and steatosis were detected in hepatocytes from all 38 livers sampled. The expression of CB1 was more marked in hepatocytes localized next to portal triads. Near the central veins, the expression was significantly weaker. Steatosis was more marked in areas of increased CB1 overexpres with publications reporting that CB1 in hepatocytes increases lipogenesis and contributes to inflammation.</p>},
  author       = {Zduniak, Krzysztof and Ziółkowski, Piotr and Regnell, Pontus and Tollet-Egnell, Petra and Åkesson, Lina and Cooper, Martin E.},
  issn         = {1792-0981},
  keyword      = {Cannabinoid receptor 1,Fatty liver,Immunohistochemistry,Rats},
  language     = {eng},
  month        = {04},
  number       = {4},
  pages        = {1227--1230},
  publisher    = {ARRAY(0xb0276a8)},
  series       = {Experimental and Therapeutic Medicine},
  title        = {Immunohistochemical analysis of cannabinoid receptor 1 expression in steatotic rat livers},
  url          = {http://dx.doi.org/10.3892/etm.2016.3036},
  volume       = {11},
  year         = {2016},
}