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Vaginal lactobacillus flora of healthy Swedish women.

Vasquez, Alejandra LU ; Jakobsson, Tell; Ahrné, Siv LU ; Forsum, Urban and Molin, Göran LU (2002) In Journal of Clinical Microbiology 40(8). p.2746-2749
Abstract
Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel... (More)
Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel electrophoresis, multiplex PCR, and 16S ribosomal DNA sequencing. The vaginal flora of most participants was dominated by a single RAPD type, but five of them harbored two RAPD types representing two different species or strains. The most frequently occurring species were Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. L. iners has not previously been reported as one of the predominant Lactobacillus species in the vagina. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
40
issue
8
pages
2746 - 2749
publisher
American Society for Microbiology
external identifiers
  • PMID:12149323
  • WOS:000177255900006
  • Scopus:0036702243
ISSN
1098-660X
DOI
10.1128/JCM.40.8.2746-2749.2002
language
English
LU publication?
yes
id
9fc41bc6-5933-4f11-a103-3f8ca84513a5 (old id 109681)
date added to LUP
2007-07-19 11:25:35
date last changed
2016-11-13 04:27:05
@misc{9fc41bc6-5933-4f11-a103-3f8ca84513a5,
  abstract     = {Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel electrophoresis, multiplex PCR, and 16S ribosomal DNA sequencing. The vaginal flora of most participants was dominated by a single RAPD type, but five of them harbored two RAPD types representing two different species or strains. The most frequently occurring species were Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. L. iners has not previously been reported as one of the predominant Lactobacillus species in the vagina.},
  author       = {Vasquez, Alejandra and Jakobsson, Tell and Ahrné, Siv and Forsum, Urban and Molin, Göran},
  issn         = {1098-660X},
  language     = {eng},
  number       = {8},
  pages        = {2746--2749},
  publisher    = {ARRAY(0x7fd7640)},
  series       = {Journal of Clinical Microbiology},
  title        = {Vaginal lactobacillus flora of healthy Swedish women.},
  url          = {http://dx.doi.org/10.1128/JCM.40.8.2746-2749.2002},
  volume       = {40},
  year         = {2002},
}