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High GAD65 autoantibody levels in nondiabetic adults are associated with HLA but not with CTLA-4 or INS VNTR.

Rolandsson, O; Hägg, E; Janer, M; Rutledge, E; Gaur, L K; Nilsson, M; Hallmans, G and Lernmark, Åke LU (2003) In Journal of Internal Medicine 253(4). p.447-453
Abstract
OBJECTIVES: To explore the relationship between genetic background and antibody levels in a nondiabetic population. We evaluated if high levels of autoantibodies against the 65 kDa isoform of glutamic acid decarboxylase (GAD65Ab), were associated with high-risk genes, i.e. HLA, CTLA-4 and INS VNTR genes. DESIGN AND SUBJECTS: Seventy-five (M/F 39/36) subjects exceeding the 95th percentile of GAD65 autoantibody index and 75 age and sex matched subjects below the 95th percentile, randomly selected amongst participants in the Västerbotten Intervention Programme. METHODS: The GAD65 Ab were measured in a radioligand-binding assay. HLA class II typing was performed by an oligoblot hybridization method. CTLA-4 repeat length was analysed and... (More)
OBJECTIVES: To explore the relationship between genetic background and antibody levels in a nondiabetic population. We evaluated if high levels of autoantibodies against the 65 kDa isoform of glutamic acid decarboxylase (GAD65Ab), were associated with high-risk genes, i.e. HLA, CTLA-4 and INS VNTR genes. DESIGN AND SUBJECTS: Seventy-five (M/F 39/36) subjects exceeding the 95th percentile of GAD65 autoantibody index and 75 age and sex matched subjects below the 95th percentile, randomly selected amongst participants in the Västerbotten Intervention Programme. METHODS: The GAD65 Ab were measured in a radioligand-binding assay. HLA class II typing was performed by an oligoblot hybridization method. CTLA-4 repeat length was analysed and divided into short forms and long forms. Class I and class III alleles of INS VNTR were detected. Differences in distribution were tested by Pearson chi-square with Yates correction. Odds ratios (OR) were used to compare groups calculated with Cochran's and (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Internal Medicine
volume
253
issue
4
pages
447 - 453
publisher
Wiley-Blackwell
external identifiers
  • Scopus:0345701218
ISSN
1365-2796
DOI
10.1046/j.1365-2796.2003.01115.x
language
English
LU publication?
yes
id
199d256e-5936-4c79-ae26-b0245d402fe4 (old id 1127430)
date added to LUP
2008-06-09 09:51:38
date last changed
2016-10-13 04:39:17
@misc{199d256e-5936-4c79-ae26-b0245d402fe4,
  abstract     = {OBJECTIVES: To explore the relationship between genetic background and antibody levels in a nondiabetic population. We evaluated if high levels of autoantibodies against the 65 kDa isoform of glutamic acid decarboxylase (GAD65Ab), were associated with high-risk genes, i.e. HLA, CTLA-4 and INS VNTR genes. DESIGN AND SUBJECTS: Seventy-five (M/F 39/36) subjects exceeding the 95th percentile of GAD65 autoantibody index and 75 age and sex matched subjects below the 95th percentile, randomly selected amongst participants in the Västerbotten Intervention Programme. METHODS: The GAD65 Ab were measured in a radioligand-binding assay. HLA class II typing was performed by an oligoblot hybridization method. CTLA-4 repeat length was analysed and divided into short forms and long forms. Class I and class III alleles of INS VNTR were detected. Differences in distribution were tested by Pearson chi-square with Yates correction. Odds ratios (OR) were used to compare groups calculated with Cochran's and},
  author       = {Rolandsson, O and Hägg, E and Janer, M and Rutledge, E and Gaur, L K and Nilsson, M and Hallmans, G and Lernmark, Åke},
  issn         = {1365-2796},
  language     = {eng},
  number       = {4},
  pages        = {447--453},
  publisher    = {ARRAY(0x8597710)},
  series       = {Journal of Internal Medicine},
  title        = {High GAD65 autoantibody levels in nondiabetic adults are associated with HLA but not with CTLA-4 or INS VNTR.},
  url          = {http://dx.doi.org/10.1046/j.1365-2796.2003.01115.x},
  volume       = {253},
  year         = {2003},
}