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Antibodies to GAD65 and peripheral nerve function in the DCCT.

Hoeldtke, RD; Hampe, CS; Bekris, LM; Hobbs, G; Bryner, KD; Lernmark, Åke LU and DCCT Research Group, The (2007) In J Neuroimmunol. 185(1-2). p.182-189
Abstract
Antibodies to the smaller isoform of glutamic acid decarboxylase (GAD65Ab) have been linked to the presence of neuropathy in Type 1 diabetes in several small studies. We attempted to confirm this association by measuring GAD65Ab, GAD65Ab epitopes and IA-2Ab in 511 patients who participated in the Diabetes Control and Complications Trial (DCCT). We also tested for correlations between these autoantibodies and C-peptide and glycemic control. We only included patients for whom serum was available from the first 4 years of their illness. The presence or absence of neuropathy was determined by electrophysiological studies, autonomic testing and clinical evaluation at baseline and 5 years into the trial or at close out. Samples from controls... (More)
Antibodies to the smaller isoform of glutamic acid decarboxylase (GAD65Ab) have been linked to the presence of neuropathy in Type 1 diabetes in several small studies. We attempted to confirm this association by measuring GAD65Ab, GAD65Ab epitopes and IA-2Ab in 511 patients who participated in the Diabetes Control and Complications Trial (DCCT). We also tested for correlations between these autoantibodies and C-peptide and glycemic control. We only included patients for whom serum was available from the first 4 years of their illness. The presence or absence of neuropathy was determined by electrophysiological studies, autonomic testing and clinical evaluation at baseline and 5 years into the trial or at close out. Samples from controls (patients without neuropathy at 5 years) were selected for patients who had similar C-peptide responses to a standardized meal at baseline. The GAD65Ab index correlated with HgbA1c only in the adult participants and only at baseline. The adults initially in poor control (upper tertile for glycemia) had higher GAD65Ab and lower C-peptides. The GAD65Ab index was not significantly different in patients with confirmed clinical neuropathy at 5 years versus controls matched for C-peptide (.248 ± .03 versus .278 ± .03). Epitope analysis, based on the blocking of conformational epitopes by recombinant Fab, revealed that the binding to multiple epitopes was decreased in the patients with neuropathy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
J Neuroimmunol.
volume
185
issue
1-2
pages
182 - 189
external identifiers
  • Scopus:33947690753
DOI
10.1016/j.jneuroim.2007.01.009
language
English
LU publication?
yes
id
fd8d0a0c-0620-49fc-b261-69613c2cfcd8 (old id 1140978)
date added to LUP
2008-08-14 09:27:57
date last changed
2016-10-13 04:56:24
@misc{fd8d0a0c-0620-49fc-b261-69613c2cfcd8,
  abstract     = {Antibodies to the smaller isoform of glutamic acid decarboxylase (GAD65Ab) have been linked to the presence of neuropathy in Type 1 diabetes in several small studies. We attempted to confirm this association by measuring GAD65Ab, GAD65Ab epitopes and IA-2Ab in 511 patients who participated in the Diabetes Control and Complications Trial (DCCT). We also tested for correlations between these autoantibodies and C-peptide and glycemic control. We only included patients for whom serum was available from the first 4 years of their illness. The presence or absence of neuropathy was determined by electrophysiological studies, autonomic testing and clinical evaluation at baseline and 5 years into the trial or at close out. Samples from controls (patients without neuropathy at 5 years) were selected for patients who had similar C-peptide responses to a standardized meal at baseline. The GAD65Ab index correlated with HgbA1c only in the adult participants and only at baseline. The adults initially in poor control (upper tertile for glycemia) had higher GAD65Ab and lower C-peptides. The GAD65Ab index was not significantly different in patients with confirmed clinical neuropathy at 5 years versus controls matched for C-peptide (.248 ± .03 versus .278 ± .03). Epitope analysis, based on the blocking of conformational epitopes by recombinant Fab, revealed that the binding to multiple epitopes was decreased in the patients with neuropathy.},
  author       = {Hoeldtke, RD and Hampe, CS and Bekris, LM and Hobbs, G and Bryner, KD and Lernmark, Åke and DCCT Research Group, The},
  language     = {eng},
  number       = {1-2},
  pages        = {182--189},
  series       = {J Neuroimmunol.},
  title        = {Antibodies to GAD65 and peripheral nerve function in the DCCT.},
  url          = {http://dx.doi.org/10.1016/j.jneuroim.2007.01.009},
  volume       = {185},
  year         = {2007},
}