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Inflammation regulates functional integration of neurons born in adult brain.

Jakubs, Katherine LU ; Bonde, Sara LU ; Iosif, Robert LU ; Ekdahl Clementson, Christine LU ; Kokaia, Zaal LU ; Kokaia, Merab LU and Lindvall, Olle LU (2008) In The Journal of neuroscience : the official journal of the Society for Neuroscience 28(47). p.12477-12488
Abstract
Inflammation influences several steps of adult neurogenesis, but whether it regulates the functional integration of the new neurons is unknown. Here, we explored, using confocal microscopy and whole-cell patch-clamp recordings, whether a chronic inflammatory environment affects the morphological and electrophysiological properties of new dentate gyrus granule cells, labeled with a retroviral vector encoding green fluorescent protein. Rats were exposed to intrahippocampal injection of lipopolysaccharide, which gave rise to long-lasting microglia activation. Inflammation caused no changes in intrinsic membrane properties, location, dendritic arborization, or spine density and morphology of the new cells. Excitatory synaptic drive increased... (More)
Inflammation influences several steps of adult neurogenesis, but whether it regulates the functional integration of the new neurons is unknown. Here, we explored, using confocal microscopy and whole-cell patch-clamp recordings, whether a chronic inflammatory environment affects the morphological and electrophysiological properties of new dentate gyrus granule cells, labeled with a retroviral vector encoding green fluorescent protein. Rats were exposed to intrahippocampal injection of lipopolysaccharide, which gave rise to long-lasting microglia activation. Inflammation caused no changes in intrinsic membrane properties, location, dendritic arborization, or spine density and morphology of the new cells. Excitatory synaptic drive increased to the same extent in new and mature cells in the inflammatory environment, suggesting increased network activity in hippocampal neural circuitries of lipopolysaccharide-treated animals. In contrast, inhibitory synaptic drive was more enhanced by inflammation in the new cells. Also, larger clusters of the postsynaptic GABA(A) receptor scaffolding protein gephyrin were found on dendrites of new cells born in the inflammatory environment. We demonstrate for the first time that inflammation influences the functional integration of adult-born hippocampal neurons. Our data indicate a high degree of synaptic plasticity of the new neurons in the inflammatory environment, which enables them to respond to the increase in excitatory input with a compensatory upregulation of activity and efficacy at their afferent inhibitory synapses. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of neuroscience : the official journal of the Society for Neuroscience
volume
28
issue
47
pages
12477 - 12488
publisher
Society for Neuroscience
external identifiers
  • WOS:000261191000033
  • PMID:19020040
  • Scopus:58149388884
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.3240-08.2008
language
English
LU publication?
yes
id
c6c66c6d-96d4-4ae2-baa1-194eefce1552 (old id 1271313)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19020040?dopt=Abstract
date added to LUP
2008-12-04 16:31:58
date last changed
2016-11-27 04:27:58
@misc{c6c66c6d-96d4-4ae2-baa1-194eefce1552,
  abstract     = {Inflammation influences several steps of adult neurogenesis, but whether it regulates the functional integration of the new neurons is unknown. Here, we explored, using confocal microscopy and whole-cell patch-clamp recordings, whether a chronic inflammatory environment affects the morphological and electrophysiological properties of new dentate gyrus granule cells, labeled with a retroviral vector encoding green fluorescent protein. Rats were exposed to intrahippocampal injection of lipopolysaccharide, which gave rise to long-lasting microglia activation. Inflammation caused no changes in intrinsic membrane properties, location, dendritic arborization, or spine density and morphology of the new cells. Excitatory synaptic drive increased to the same extent in new and mature cells in the inflammatory environment, suggesting increased network activity in hippocampal neural circuitries of lipopolysaccharide-treated animals. In contrast, inhibitory synaptic drive was more enhanced by inflammation in the new cells. Also, larger clusters of the postsynaptic GABA(A) receptor scaffolding protein gephyrin were found on dendrites of new cells born in the inflammatory environment. We demonstrate for the first time that inflammation influences the functional integration of adult-born hippocampal neurons. Our data indicate a high degree of synaptic plasticity of the new neurons in the inflammatory environment, which enables them to respond to the increase in excitatory input with a compensatory upregulation of activity and efficacy at their afferent inhibitory synapses.},
  author       = {Jakubs, Katherine and Bonde, Sara and Iosif, Robert and Ekdahl Clementson, Christine and Kokaia, Zaal and Kokaia, Merab and Lindvall, Olle},
  issn         = {1529-2401},
  language     = {eng},
  number       = {47},
  pages        = {12477--12488},
  publisher    = {ARRAY(0x7c93c38)},
  series       = {The Journal of neuroscience : the official journal of the Society for Neuroscience},
  title        = {Inflammation regulates functional integration of neurons born in adult brain.},
  url          = {http://dx.doi.org/10.1523/JNEUROSCI.3240-08.2008},
  volume       = {28},
  year         = {2008},
}