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The non-coding RNA of the multidrug resistance-linked vault particle encodes multiple regulatory small RNAs.

Persson, Helena LU ; Kvist, Anders LU ; Vallon-Christersson, Johan LU ; Medstrand, Patrik LU ; Borg, Åke LU and Rovira, Carlos LU (2009) In Nature Cell Biology 11(10). p.1268-1271
Abstract
Vault particles are conserved organelles implicated in multidrug resistance and intracellular transport. They contain three different proteins and non-coding vault RNAs (vRNAs). Here we show that human vRNAs produce several small RNAs (svRNAs) by mechanisms different from those in the canonical microRNA (miRNA) pathway. At least one of these svRNAs, svRNAb, associates with Argonaute proteins to guide sequence-specific cleavage and regulate gene expression similarly to miRNAs. We demonstrate that svRNAb downregulates CYP3A4, a key enzyme in drug metabolism. Our findings expand the repertoire of small regulatory RNAs and assign, for the first time, a function to vRNAs that may help explain the association between vault particles and drug... (More)
Vault particles are conserved organelles implicated in multidrug resistance and intracellular transport. They contain three different proteins and non-coding vault RNAs (vRNAs). Here we show that human vRNAs produce several small RNAs (svRNAs) by mechanisms different from those in the canonical microRNA (miRNA) pathway. At least one of these svRNAs, svRNAb, associates with Argonaute proteins to guide sequence-specific cleavage and regulate gene expression similarly to miRNAs. We demonstrate that svRNAb downregulates CYP3A4, a key enzyme in drug metabolism. Our findings expand the repertoire of small regulatory RNAs and assign, for the first time, a function to vRNAs that may help explain the association between vault particles and drug resistance. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Cell Biology
volume
11
issue
10
pages
1268 - 1271
publisher
Nature Publishing Group
external identifiers
  • WOS:000270382000018
  • PMID:19749744
  • Scopus:70349652191
ISSN
1465-7392
DOI
10.1038/ncb1972
project
CREATE Health
language
English
LU publication?
yes
id
1a267a54-1563-4360-8753-0ea975cdc78f (old id 1483540)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19749744?dopt=Abstract
date added to LUP
2009-10-07 10:52:18
date last changed
2016-11-24 15:13:31
@misc{1a267a54-1563-4360-8753-0ea975cdc78f,
  abstract     = {Vault particles are conserved organelles implicated in multidrug resistance and intracellular transport. They contain three different proteins and non-coding vault RNAs (vRNAs). Here we show that human vRNAs produce several small RNAs (svRNAs) by mechanisms different from those in the canonical microRNA (miRNA) pathway. At least one of these svRNAs, svRNAb, associates with Argonaute proteins to guide sequence-specific cleavage and regulate gene expression similarly to miRNAs. We demonstrate that svRNAb downregulates CYP3A4, a key enzyme in drug metabolism. Our findings expand the repertoire of small regulatory RNAs and assign, for the first time, a function to vRNAs that may help explain the association between vault particles and drug resistance.},
  author       = {Persson, Helena and Kvist, Anders and Vallon-Christersson, Johan and Medstrand, Patrik and Borg, Åke and Rovira, Carlos},
  issn         = {1465-7392},
  language     = {eng},
  number       = {10},
  pages        = {1268--1271},
  publisher    = {ARRAY(0x7f87118)},
  series       = {Nature Cell Biology},
  title        = {The non-coding RNA of the multidrug resistance-linked vault particle encodes multiple regulatory small RNAs.},
  url          = {http://dx.doi.org/10.1038/ncb1972},
  volume       = {11},
  year         = {2009},
}