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Design and development of electrochemical sensors with application in bioanalysis

Zor, Kinga LU (2011)
Abstract (Swedish)
Popular Abstract in English

There is a continuous search for reliable, robust easy to use, portable and cost effective

analytical tools with applications in food industry, biomedicine, environmental

monitoring and homeland security.

The main focus of the thesis was on the development of electrochemical (bio)sensors

designed to detect analytes of key importance from biological matrixes (e.g.,

fermentation broth, meat samples and cell culture media) applied in two major fields of

interest: food & beverage industry and biomedical research.

Calpastatin, a meat tenderness biomarker, was successfully detected, from meat samples,

with the developed label... (More)
Popular Abstract in English

There is a continuous search for reliable, robust easy to use, portable and cost effective

analytical tools with applications in food industry, biomedicine, environmental

monitoring and homeland security.

The main focus of the thesis was on the development of electrochemical (bio)sensors

designed to detect analytes of key importance from biological matrixes (e.g.,

fermentation broth, meat samples and cell culture media) applied in two major fields of

interest: food & beverage industry and biomedical research.

Calpastatin, a meat tenderness biomarker, was successfully detected, from meat samples,

with the developed label free capacitive immunosensors and amperometric

immunoassays. Based on the amperometric immunoassay an electrochemical detection

system has been designed, fabricated and applied for calpastatin quantification and the

obtained values were correlated with Warner Bratzler Shear Force data, a routinely used

method for meat tenderness determination. Glucose and ethanol were simultaneously

monitored during a fermentation process with miniaturized enzyme based amperometric

biosensors connected to a microdialysis probe.

Beside the application in food analysis, it was shown the possibility of application of

amperometric immunoassay for calpastatin and μ-calpain in biomedicine, both proteins

having an important role in the pathology of several diseases. Electrochemical cellular

dynamics monitoring has been achieved in real time by using a microfluidic cell culture

system where the electrodes were integrated at the bottom of the cell culture chambers. (Less)
Abstract
There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security.

The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food & beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media.

The proposed monitoring methods for calpastatin (a meat tenderness biomarker), successfully applied to meat samples, were based on (i) label free capacitive immunosensors and (ii)amperometric... (More)
There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security.

The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food & beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media.

The proposed monitoring methods for calpastatin (a meat tenderness biomarker), successfully applied to meat samples, were based on (i) label free capacitive immunosensors and (ii)amperometric immunoassays. The amperometric immunoassay approach was considered to be used when an electrochemical detection system has been designed, fabricated and applied

for calpastatin quantification from bovine skeletal muscle. The obtained values were correlated with Warner Bratzler Shear Force data, a routinely used method for meat tenderness determination. Glucose and ethanol were simultaneously monitored during a fermentation process with miniaturized enzyme based amperometric biosensors connected to a microdialysis probe.

Besides food analysis applications, the amperometric immunoassay for calpastatin and additionally for μ-calpain detection is very important in biomedicine, both proteins having an important role in the pathology of several diseases. Electrochemical cellular dynamics monitoring has been achieved in real time by using a microfluidic cell culture system, where

the electrodes were integrated at the bottom of the cell culture chambers. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Prof. Domínguez, Elena, Department of Analytical Chemistry, University of Alcala, Madrid, Spain
organization
publishing date
type
Thesis
publication status
published
subject
keywords
bioanalysis, electrochemistry, sensor, biosensor, miniaturization, real sample, immunoassay, μTAS
defense location
Lecture hall B, Centre of Chemistry and Chemical Engineering, Sölvegatan 3, Lund University Faculty of Engineering
defense date
2011-10-29 10:15
language
English
LU publication?
yes
id
b6d2b879-0d6d-4b66-90f2-6bdd9f83a6de (old id 2168113)
date added to LUP
2011-10-05 08:18:27
date last changed
2016-09-19 08:45:19
@misc{b6d2b879-0d6d-4b66-90f2-6bdd9f83a6de,
  abstract     = {There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security.<br/><br>
The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food &amp; beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media.<br/><br>
The proposed monitoring methods for calpastatin (a meat tenderness biomarker), successfully applied to meat samples, were based on (i) label free capacitive immunosensors and (ii)amperometric immunoassays. The amperometric immunoassay approach was considered to be used when an electrochemical detection system has been designed, fabricated and applied<br/><br>
for calpastatin quantification from bovine skeletal muscle. The obtained values were correlated with Warner Bratzler Shear Force data, a routinely used method for meat tenderness determination. Glucose and ethanol were simultaneously monitored during a fermentation process with miniaturized enzyme based amperometric biosensors connected to a microdialysis probe.<br/><br>
Besides food analysis applications, the amperometric immunoassay for calpastatin and additionally for μ-calpain detection is very important in biomedicine, both proteins having an important role in the pathology of several diseases. Electrochemical cellular dynamics monitoring has been achieved in real time by using a microfluidic cell culture system, where<br/><br>
the electrodes were integrated at the bottom of the cell culture chambers.},
  author       = {Zor, Kinga},
  keyword      = {bioanalysis,electrochemistry,sensor,biosensor,miniaturization,real sample,immunoassay,μTAS},
  language     = {eng},
  title        = {Design and development of electrochemical sensors with application in bioanalysis},
  year         = {2011},
}