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New Set-up for Capillary Isoelectric Focusing in Uncoated Capillaries

Kilár, Ferenc; Végvári, Ákos LU and Mód, Andor (1998) In Journal of Chromatography A 813(2). p.349-360
Abstract
Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the... (More)
Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the analysis of hemoglobin variants. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Isoelectric focusing, Sandwich injection, Injection methods, Haemoglobin, Dyes, Nitrophenols
in
Journal of Chromatography A
volume
813
issue
2
pages
349 - 360
publisher
Elsevier
external identifiers
  • Scopus:0344541992
ISSN
0021-9673
DOI
10.1016/S0021-9673(98)00377-X
language
English
LU publication?
no
id
0934bc21-a74a-4ecb-b0bb-8cc975fecb47 (old id 3516344)
date added to LUP
2013-02-25 12:01:00
date last changed
2016-10-13 04:36:16
@misc{0934bc21-a74a-4ecb-b0bb-8cc975fecb47,
  abstract     = {Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the analysis of hemoglobin variants.},
  author       = {Kilár, Ferenc and Végvári, Ákos and Mód, Andor},
  issn         = {0021-9673},
  keyword      = {Isoelectric focusing,Sandwich injection,Injection methods,Haemoglobin,Dyes,Nitrophenols},
  language     = {eng},
  number       = {2},
  pages        = {349--360},
  publisher    = {ARRAY(0x7b06b88)},
  series       = {Journal of Chromatography A},
  title        = {New Set-up for Capillary Isoelectric Focusing in Uncoated Capillaries},
  url          = {http://dx.doi.org/10.1016/S0021-9673(98)00377-X},
  volume       = {813},
  year         = {1998},
}