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Functional analysis of the mouse J chain

Erlandsson, Lena LU (1999)
Abstract
We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct... (More)
We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct relationship between mucosal transport of secretory IgA and intestinal immune protection. A second mouse was generated where the Diphtheria toxin A gene was introduced into the J chain locus, thereby ablating J chain expressing cells. Evidence was found for the existence of two separate plasma cell populations distinguishable by the absence or the presence of J chain. The dogma that in mice the J chain is expressed in all plasma cells irrespective of isotype has been challenged. (Less)
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author
opponent
  • Dr Sideras, Paschalis, Umeå University, Sweden
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Immunology, diphtheria toxin A, plasma cell, mucosa, J chain, knock-out, serology, transplantation, Immunologi, serologi
pages
140 pages
publisher
Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden
defense location
Sölvegatan 21, Lund
defense date
1999-05-05 10:15
external identifiers
  • Other:ISRN: LUMEDW/MECM--99/1026--SE
ISBN
91-628-3544-0
language
English
LU publication?
yes
id
396d3d5f-8d01-4a2e-8303-42bd168b2cab (old id 39490)
date added to LUP
2007-06-20 15:41:44
date last changed
2016-09-19 08:45:06
@misc{396d3d5f-8d01-4a2e-8303-42bd168b2cab,
  abstract     = {We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct relationship between mucosal transport of secretory IgA and intestinal immune protection. A second mouse was generated where the Diphtheria toxin A gene was introduced into the J chain locus, thereby ablating J chain expressing cells. Evidence was found for the existence of two separate plasma cell populations distinguishable by the absence or the presence of J chain. The dogma that in mice the J chain is expressed in all plasma cells irrespective of isotype has been challenged.},
  author       = {Erlandsson, Lena},
  isbn         = {91-628-3544-0},
  keyword      = {Immunology,diphtheria toxin A,plasma cell,mucosa,J chain,knock-out,serology,transplantation,Immunologi,serologi},
  language     = {eng},
  pages        = {140},
  publisher    = {ARRAY(0x9abdd28)},
  title        = {Functional analysis of the mouse J chain},
  year         = {1999},
}