Chemical characterization of the predominant proteins secreted by mouse seminal vesicles
Lundwall, Åke LU ; Peter, A. ; Lövgren, J. ; Lilja, Hans LU
and Malm, J.
(1997)
In Eur J Biochem
249(1).
p.39-44
- Abstract
- Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein... (More)
- Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein to human semenogelins, despite the vast difference in primary structure. As previously reported for other seminal-vesicle-secreted proteins, the semenoclotin transcripts are shown to be under androgen control. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965273
- author
- Lundwall, Åke
LU
; Peter, A.
; Lövgren, J.
; Lilja, Hans
LU
and Malm, J.
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- RNA, Proteins/*chemistry/genetics/*secretion, Post-Translational, Protein Processing, Molecular Weight, Inbred BALB C, Mice, Male, Humans, Gonadal Steroid Hormones/chemistry/genetics, Cross-Linking Reagents, Amino Acid Sequence, Animals, Messenger/genetics/metabolism, Research Support, Non-U.S. Gov't, *Seminal Vesicle Secretory Proteins, Seminal Vesicles/chemistry/drug effects/*secretion, Testosterone/pharmacology, Transglutaminases
- in
- Eur J Biochem
- volume
- 249
- issue
- 1
- pages
- 39 - 44
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0030819922
- language
- English
- LU publication?
- yes
- additional info
- 1
- id
- 2fd412b7-59a1-4eda-8896-5f4707fd633e (old id 3965273)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9363751
- date added to LUP
- 2016-04-04 13:59:34
- date last changed
- 2022-01-30 01:18:01
@article{2fd412b7-59a1-4eda-8896-5f4707fd633e,
abstract = {{Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein to human semenogelins, despite the vast difference in primary structure. As previously reported for other seminal-vesicle-secreted proteins, the semenoclotin transcripts are shown to be under androgen control.}},
author = {{Lundwall, Åke and Peter, A. and Lövgren, J. and Lilja, Hans and Malm, J.}},
keywords = {{RNA; Proteins/*chemistry/genetics/*secretion; Post-Translational; Protein Processing; Molecular Weight; Inbred BALB C; Mice; Male; Humans; Gonadal Steroid Hormones/chemistry/genetics; Cross-Linking Reagents; Amino Acid Sequence; Animals; Messenger/genetics/metabolism; Research Support; Non-U.S. Gov't; *Seminal Vesicle Secretory Proteins; Seminal Vesicles/chemistry/drug effects/*secretion; Testosterone/pharmacology; Transglutaminases}},
language = {{eng}},
number = {{1}},
pages = {{39--44}},
publisher = {{Wiley-Blackwell}},
series = {{Eur J Biochem}},
title = {{Chemical characterization of the predominant proteins secreted by mouse seminal vesicles}},
url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9363751}},
volume = {{249}},
year = {{1997}},
}