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Evolution of hordein gene organization in three Hordeum species.

Pelger, Susanne LU ; Säll, Torbjörn LU and Bengtsson, Bengt Olle LU (1993) In Hereditas 119(3). p.219-231
Abstract
The inheritance pattern of hordein, the seed storage protein in barley, has been studied in two wild Hordeum species, H. murinum and H. pusillum. Three different diploid populations of each species were crossed, and the F1 plants were self-pollinated. The seeds with an F2 genotype were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several differences could be observed in the banding patterns of the parental populations. The analysis of recombinant banding patterns showed that in H. murinum there are at least four segregating loci, of which two code for B- and two for C-hordein. All the loci are linked on the same chromosome. In H. pusillum at least six segregating loci were found, of which three code for... (More)
The inheritance pattern of hordein, the seed storage protein in barley, has been studied in two wild Hordeum species, H. murinum and H. pusillum. Three different diploid populations of each species were crossed, and the F1 plants were self-pollinated. The seeds with an F2 genotype were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several differences could be observed in the banding patterns of the parental populations. The analysis of recombinant banding patterns showed that in H. murinum there are at least four segregating loci, of which two code for B- and two for C-hordein. All the loci are linked on the same chromosome. In H. pusillum at least six segregating loci were found, of which three code for B and three for C-hordein. Five of the loci are linked, while the sixth showed independent segregation. The organization of the hordein genes differs not only between these two species but also between them and the different forms of H. vulgare, as well as with other species belonging to the tribe Triticeae. Extensive rearrangements must obviously have taken place among the members of the hordein gene family since the divergence of the species in the Hordeum genus. The possibility is discussed that the genes have been moved through transposition, a possible mechanism for the physical divergence of tandemly repeated sequences. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Hereditas
volume
119
issue
3
pages
219 - 231
publisher
Wiley-Blackwell
external identifiers
  • Scopus:0027753094
ISSN
1601-5223
DOI
10.1111/j.1601-5223.1993.00219.x
language
English
LU publication?
yes
id
0408afbc-5878-4427-af7d-e4f97057e2cf (old id 4053520)
date added to LUP
2013-09-23 09:14:10
date last changed
2016-10-13 04:44:22
@misc{0408afbc-5878-4427-af7d-e4f97057e2cf,
  abstract     = {The inheritance pattern of hordein, the seed storage protein in barley, has been studied in two wild Hordeum species, H. murinum and H. pusillum. Three different diploid populations of each species were crossed, and the F1 plants were self-pollinated. The seeds with an F2 genotype were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several differences could be observed in the banding patterns of the parental populations. The analysis of recombinant banding patterns showed that in H. murinum there are at least four segregating loci, of which two code for B- and two for C-hordein. All the loci are linked on the same chromosome. In H. pusillum at least six segregating loci were found, of which three code for B and three for C-hordein. Five of the loci are linked, while the sixth showed independent segregation. The organization of the hordein genes differs not only between these two species but also between them and the different forms of H. vulgare, as well as with other species belonging to the tribe Triticeae. Extensive rearrangements must obviously have taken place among the members of the hordein gene family since the divergence of the species in the Hordeum genus. The possibility is discussed that the genes have been moved through transposition, a possible mechanism for the physical divergence of tandemly repeated sequences.},
  author       = {Pelger, Susanne and Säll, Torbjörn and Bengtsson, Bengt Olle},
  issn         = {1601-5223},
  language     = {eng},
  number       = {3},
  pages        = {219--231},
  publisher    = {ARRAY(0x9e3f8a8)},
  series       = {Hereditas},
  title        = {Evolution of hordein gene organization in three Hordeum species.},
  url          = {http://dx.doi.org/10.1111/j.1601-5223.1993.00219.x},
  volume       = {119},
  year         = {1993},
}