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Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli

de Maré, Lena LU ; Velut, Stéphane LU ; Briechle, Sebastian ; Wennerberg, Christina LU ; Cimander, Christian ; Ramchuran, Santosh LU ; Tunert, Pernilla ; Silfversparre, Gustav ; Holst, Olle LU and Hagander, Per LU (2004) p.73-79
Abstract
A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
temperature control, Escherichia coli, Fermentation process, feeding strategy
host publication
Computer Applications in Biotechnology 2004
editor
Pons, Marie-Noelle and Van Impe, Jan
pages
73 - 79
publisher
International Federation of Automatic Control
external identifiers
  • scopus:85083219082
ISBN
0-08-044251-X
language
English
LU publication?
yes
id
19b6978f-b459-4c38-bc7b-eb5617f317cc (old id 535975)
date added to LUP
2016-04-04 10:53:15
date last changed
2022-01-29 20:58:36
@inproceedings{19b6978f-b459-4c38-bc7b-eb5617f317cc,
  abstract     = {{A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.}},
  author       = {{de Maré, Lena and Velut, Stéphane and Briechle, Sebastian and Wennerberg, Christina and Cimander, Christian and Ramchuran, Santosh and Tunert, Pernilla and Silfversparre, Gustav and Holst, Olle and Hagander, Per}},
  booktitle    = {{Computer Applications in Biotechnology 2004}},
  editor       = {{Pons, Marie-Noelle and Van Impe, Jan}},
  isbn         = {{0-08-044251-X}},
  keywords     = {{temperature control; Escherichia coli; Fermentation process; feeding strategy}},
  language     = {{eng}},
  pages        = {{73--79}},
  publisher    = {{International Federation of Automatic Control}},
  title        = {{Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli}},
  year         = {{2004}},
}