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Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

Lohmander, Stefan LU (1972) In Biochimica et Biophysica Acta. General Subjects 264(3). p.411-417
Abstract

A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined. © 1972.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochimica et Biophysica Acta. General Subjects
volume
264
issue
3
pages
7 pages
publisher
Elsevier
external identifiers
  • Scopus:0015512384
ISSN
0304-4165
DOI
10.1016/0304-4165(72)90003-7
language
English
LU publication?
yes
id
586a4d48-6d98-4dda-aab3-ca3ab2befddc
date added to LUP
2016-05-04 12:36:47
date last changed
2016-07-04 10:44:04
@misc{586a4d48-6d98-4dda-aab3-ca3ab2befddc,
  abstract     = {<p>A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined. © 1972.</p>},
  author       = {Lohmander, Stefan},
  issn         = {0304-4165},
  language     = {eng},
  month        = {05},
  number       = {3},
  pages        = {411--417},
  publisher    = {ARRAY(0x8397a10)},
  series       = {Biochimica et Biophysica Acta. General Subjects},
  title        = {Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay},
  url          = {http://dx.doi.org/10.1016/0304-4165(72)90003-7},
  volume       = {264},
  year         = {1972},
}